Shim Youn Young, Mustafa Rana, Shen Jianheng, Ratanapariyanuch Kornsulee, Reaney Martin J T
Department of Plant Sciences, University of Saskatchewan; Prairie Tide Chemicals Inc.; Guangdong Saskatchewan Oilseed Joint Laboratory (GUSTO), Department of Food Science and Engineering, Jinan University.
Department of Plant Sciences, University of Saskatchewan.
J Vis Exp. 2018 Feb 10(132):56305. doi: 10.3791/56305.
Chickpea and other pulses are commonly sold as canned products packed in a thick solution or a brine. This solution has recently been shown to produce stable foams and emulsions, and can act as a thickener. Recently interest in this product has been enhanced through the internet where it is proposed that this solution, now called aquafaba by a growing community, can be used a replacement for egg and milk protein. As aquafaba is both new and being developed by an internet based community little is known of its composition or properties. Aquafaba was recovered from 10 commercial canned chickpea products and correlations among aquafaba composition, density, viscosity and foaming properties were investigated. Proton NMR was used to characterize aquafaba composition before and after ultrafiltration through membranes with different molecular weight cut offs (MWCOs of 3, 10, or 50 kDa). A protocol for electrophoresis, and peptide mass fingerprinting is also presented. Those methods provided valuable information regarding components responsible for aquafaba functional properties. This information will allow the development of practices to produce standard commercial aquafaba products and may help consumers select products of superior or consistent utility.
鹰嘴豆和其他豆类通常以罐装产品形式出售,包装在浓稠溶液或盐水中。最近研究表明,这种溶液能产生稳定的泡沫和乳液,还可作为增稠剂。近来,通过互联网,人们对该产品的兴趣有所增加,在网上有人提出,这种现在被越来越多的人称为“aquafaba”的溶液可替代鸡蛋和牛奶蛋白。由于aquafaba是一种新出现且由网络群体开发的产品,人们对其成分和特性知之甚少。从10种市售罐装鹰嘴豆产品中提取了aquafaba,并研究了aquafaba成分、密度、粘度和发泡特性之间的相关性。通过不同截留分子量(3 kDa、10 kDa或50 kDa)的膜进行超滤前后,利用质子核磁共振对aquafaba成分进行了表征。还介绍了一种电泳和肽质量指纹图谱分析方法。这些方法为了解aquafaba功能特性的相关成分提供了有价值的信息。这些信息将有助于制定生产标准商业aquafaba产品的方法,也可能帮助消费者选择效用更佳或更稳定的产品。