从稀酒糟中生产蛋白质浓缩物
Protein Concentrate Production from Thin Stillage.
作者信息
Ratanapariyanuch Kornsulee, Shim Youn Young, Emami Shahram, Reaney Martin J T
机构信息
Department of Plant Sciences, University of Saskatchewan , 51 Campus Drive, Saskatoon, Saskatchewan S7N 5A8, Canada.
Prairie Tide Chemicals Inc. , 102 Melville Street, Saskatoon, Saskatchewan S7J 0R1, Canada.
出版信息
J Agric Food Chem. 2016 Dec 21;64(50):9488-9496. doi: 10.1021/acs.jafc.6b03816. Epub 2016 Dec 12.
Two-stage fermentation (TSF) of saccharified wheat with a consortium of endemic lactobacilli produced CO and induced colloid separation of fermented solution to produce a protein concentrate (PC). Protein-rich slurry (50%, db) was obtained by decanting solution or skimming floating material during or after TSF. Washing and drying processes were explored to improve protein content, extend storage life of slurry, and yield converted stillage for compound recovery. Centrifuging and washing slurry afforded a PC and clarified solution. PC protein content increased to 60% (w/w, db). The PC was dried in a spray dryer or drum dryer or tray dryer. Dried PC water activity ranged 0.23-0.30. The dried PC lysine content was low, but lysine availability (95%) was excellent. Liquid from TSF and washing was readily microfiltered. Mass recovery of protein, glycerol, 1,3-propanediol, lactic acid, acetic acid, and glycerylphosphorylcholine from combined TSF, washing, and filtration were 66, 76, 72, 77, 74, and 84%, respectively.
用本地乳酸菌 consortium 对糖化小麦进行两阶段发酵（TSF）产生了 CO，并促使发酵液进行胶体分离以生产蛋白质浓缩物（PC）。在TSF期间或之后，通过倾析溶液或撇去漂浮物获得了富含蛋白质的浆料（50%，干基）。探索了洗涤和干燥工艺，以提高蛋白质含量、延长浆料的储存寿命，并产出用于化合物回收的转化酒糟。对浆料进行离心和洗涤得到了PC和澄清溶液。PC的蛋白质含量提高到了60%（w/w，干基）。PC在喷雾干燥器、滚筒干燥器或托盘干燥器中进行干燥。干燥后的PC水分活度范围为0.23 - 0.30。干燥后的PC赖氨酸含量较低，但赖氨酸利用率（95%）极佳。来自TSF和洗涤的液体易于进行微滤。从TSF、洗涤和过滤的组合过程中，蛋白质、甘油、1,3 - 丙二醇、乳酸、乙酸和甘油磷酸胆碱的质量回收率分别为66%、76%、72%、77%、74%和84%。
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