一种评估流感血凝素特异性抗体诱导的Fc介导效应功能的方法。
A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies.
作者信息
Bailey Mark J, Broecker Felix, Leon Paul E, Tan Gene S
机构信息
Department of Microbiology, Icahn School of Medicine at Mount Sinai; Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai.
Department of Microbiology, Icahn School of Medicine at Mount Sinai.
出版信息
J Vis Exp. 2018 Feb 23(132):56256. doi: 10.3791/56256.
Abstract
Antibodies play a crucial role in coupling the innate and adaptive immune responses against viral pathogens through their antigen binding domains and Fc-regions. Here, we describe how to measure the activation of Fc effector functions by monoclonal antibodies targeting the influenza virus hemagglutinin with the use of a genetically engineered Jurkat cell line expressing an activating type 1 Fc-FcγR. Using this method, the contribution of specific Fc-FcγR interactions conferred by immunoglobulins can be determined using an in vitro assay.
摘要
抗体通过其抗原结合域和Fc区域在针对病毒病原体的固有免疫和适应性免疫反应的耦合中发挥关键作用。在此，我们描述了如何使用表达激活型1 Fc-FcγR的基因工程Jurkat细胞系，通过靶向流感病毒血凝素的单克隆抗体来测量Fc效应器功能的激活。使用这种方法，可以通过体外试验确定免疫球蛋白赋予的特定Fc-FcγR相互作用的贡献。
相似文献
1
A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies.一种评估流感血凝素特异性抗体诱导的Fc介导效应功能的方法。
J Vis Exp. 2018 Feb 23(132):56256. doi: 10.3791/56256.
2
Broadly Neutralizing Hemagglutinin Stalk-Specific Antibodies Induce Potent Phagocytosis of Immune Complexes by Neutrophils in an Fc-Dependent Manner.广泛中和的血凝素茎特异性抗体以Fc依赖的方式诱导中性粒细胞对免疫复合物进行有效的吞噬作用。
mBio. 2016 Oct 4;7(5):e01624-16. doi: 10.1128/mBio.01624-16.
3
Optimal activation of Fc-mediated effector functions by influenza virus hemagglutinin antibodies requires two points of contact.流感病毒血凝素抗体对Fc介导的效应功能的最佳激活需要两个接触点。
Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):E5944-E5951. doi: 10.1073/pnas.1613225113. Epub 2016 Sep 19.
4
Divergent Requirement of Fc-Fcγ Receptor Interactions for Protection against Influenza Viruses by Two Pan-H5 Hemagglutinin Antibodies.两种泛H5血凝素抗体通过Fc-Fcγ受体相互作用对流感病毒产生保护作用的不同需求
J Virol. 2017 May 12;91(11). doi: 10.1128/JVI.02065-16. Print 2017 Jun 1.
5
Simultaneous exposure to FcγR and FcαR on monocytes and macrophages enhances antitumor activity in vivo.单核细胞和巨噬细胞上的FcγR和FcαR同时暴露可增强体内抗肿瘤活性。
Oncotarget. 2017 Jun 13;8(24):39356-39366. doi: 10.18632/oncotarget.17000.
6
Hierarchical and Redundant Roles of Activating FcγRs in Protection against Influenza Disease by M2e-Specific IgG1 and IgG2a Antibodies.激活型FcγR在M2e特异性IgG1和IgG2a抗体预防流感疾病中的分层和冗余作用
J Virol. 2017 Mar 13;91(7). doi: 10.1128/JVI.02500-16. Print 2017 Apr 1.
7
Fc-Fusion Drugs Have FcγR/C1q Binding and Signaling Properties That May Affect Their Immunogenicity.Fc 融合药物具有 FcγR/C1q 结合和信号转导特性，这些特性可能会影响它们的免疫原性。
AAPS J. 2019 May 6;21(4):62. doi: 10.1208/s12248-019-0336-8.
8
Epitope specificity plays a critical role in regulating antibody-dependent cell-mediated cytotoxicity against influenza A virus.表位特异性在调节针对甲型流感病毒的抗体依赖性细胞介导的细胞毒性中起着关键作用。
Proc Natl Acad Sci U S A. 2016 Oct 18;113(42):11931-11936. doi: 10.1073/pnas.1609316113. Epub 2016 Oct 3.
9
Role of Fc-FcγR interactions in the antitumor activity of therapeutic antibodies.Fc-FcγR相互作用在治疗性抗体抗肿瘤活性中的作用。
Immunol Cell Biol. 2017 Apr;95(4):340-346. doi: 10.1038/icb.2016.121. Epub 2016 Dec 15.
10
Diversification of IgG effector functions.IgG 效应功能的多样化。
Int Immunol. 2017 Jul 1;29(7):303-310. doi: 10.1093/intimm/dxx025.
引用本文的文献
1
Age-associated defect in ADCC response to COVID-19 vaccine.与年龄相关的对新冠疫苗的抗体依赖的细胞介导的细胞毒性反应缺陷
NPJ Vaccines. 2025 Jul 1;10(1):132. doi: 10.1038/s41541-025-01196-9.
2
Humoral responses to SARS-CoV-2 vaccine in vasculitis-related immune suppression.血管炎相关免疫抑制患者对SARS-CoV-2疫苗的体液免疫反应。
Sci Adv. 2025 Feb 14;11(7):eadq3342. doi: 10.1126/sciadv.adq3342. Epub 2025 Feb 12.
3
Beyond bNAbs: Uses, Risks, and Opportunities for Therapeutic Application of Non-Neutralising Antibodies in Viral Infection.超越广谱中和抗体：非中和抗体在病毒感染治疗应用中的用途、风险与机遇
Antibodies (Basel). 2024 Apr 3;13(2):28. doi: 10.3390/antib13020028.
4
Characterization of FcγRIa (CD64) as a Ligand Molecule for Site-Specific IgG1 Capture: A Side-By-Side Comparison with Protein A.鉴定 FcγRIa（CD64）作为特异性 IgG1 捕获配体分子：与蛋白 A 的并列比较。
Langmuir. 2022 Dec 6;38(48):14623-14634. doi: 10.1021/acs.langmuir.2c02022. Epub 2022 Nov 23.
5
Extending the Stalk Enhances Immunogenicity of the Influenza Virus Neuraminidase.延长茎干可增强流感病毒神经氨酸酶的免疫原性。
J Virol. 2019 Aug 28;93(18). doi: 10.1128/JVI.00840-19. Print 2019 Sep 15.
6
A mosaic hemagglutinin-based influenza virus vaccine candidate protects mice from challenge with divergent H3N2 strains.一种基于镶嵌血凝素的流感病毒候选疫苗可保护小鼠免受不同H3N2毒株的攻击。
NPJ Vaccines. 2019 Jul 19;4:31. doi: 10.1038/s41541-019-0126-4. eCollection 2019.
本文引用的文献
1
Universal influenza virus vaccines and therapeutic antibodies.通用流感病毒疫苗和治疗性抗体。
Clin Microbiol Infect. 2017 Apr;23(4):222-228. doi: 10.1016/j.cmi.2017.02.009. Epub 2017 Feb 12.
2
Antibody-dependent cellular cytotoxicity and influenza virus.抗体依赖的细胞毒性作用与流感病毒
Curr Opin Virol. 2017 Feb;22:89-96. doi: 10.1016/j.coviro.2016.12.002. Epub 2017 Jan 11.
3
Influenza virus-specific antibody dependent cellular cytoxicity induced by vaccination or natural infection.由疫苗接种或自然感染诱导的流感病毒特异性抗体依赖性细胞毒性。
Vaccine. 2017 Jan 5;35(2):238-247. doi: 10.1016/j.vaccine.2016.11.082. Epub 2016 Nov 30.
4
Epitope specificity plays a critical role in regulating antibody-dependent cell-mediated cytotoxicity against influenza A virus.表位特异性在调节针对甲型流感病毒的抗体依赖性细胞介导的细胞毒性中起着关键作用。
Proc Natl Acad Sci U S A. 2016 Oct 18;113(42):11931-11936. doi: 10.1073/pnas.1609316113. Epub 2016 Oct 3.
5
Optimal activation of Fc-mediated effector functions by influenza virus hemagglutinin antibodies requires two points of contact.流感病毒血凝素抗体对Fc介导的效应功能的最佳激活需要两个接触点。
Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):E5944-E5951. doi: 10.1073/pnas.1613225113. Epub 2016 Sep 19.
6
Both Neutralizing and Non-Neutralizing Human H7N9 Influenza Vaccine-Induced Monoclonal Antibodies Confer Protection.中和性和非中和性人源H7N9流感疫苗诱导的单克隆抗体均具有保护作用。
Cell Host Microbe. 2016 Jun 8;19(6):800-13. doi: 10.1016/j.chom.2016.05.014.
7
Evaluation of Antihemagglutinin and Antineuraminidase Antibodies as Correlates of Protection in an Influenza A/H1N1 Virus Healthy Human Challenge Model.在甲型H1N1流感病毒健康人体激发模型中评估抗血凝素和抗神经氨酸酶抗体作为保护相关性指标的研究
mBio. 2016 Apr 19;7(2):e00417-16. doi: 10.1128/mBio.00417-16.
8
Broadly-Reactive Neutralizing and Non-neutralizing Antibodies Directed against the H7 Influenza Virus Hemagglutinin Reveal Divergent Mechanisms of Protection.针对H7流感病毒血凝素的广泛反应性中和及非中和抗体揭示了不同的保护机制。
PLoS Pathog. 2016 Apr 15;12(4):e1005578. doi: 10.1371/journal.ppat.1005578. eCollection 2016 Apr.
9
Antibody Responses with Fc-Mediated Functions after Vaccination of HIV-Infected Subjects with Trivalent Influenza Vaccine.用三价流感疫苗接种HIV感染受试者后具有Fc介导功能的抗体反应
J Virol. 2016 May 27;90(12):5724-5734. doi: 10.1128/JVI.00285-16. Print 2016 Jun 15.
10
Novel universal influenza virus vaccine approaches.新型通用流感病毒疫苗研发方法。
Curr Opin Virol. 2016 Apr;17:95-103. doi: 10.1016/j.coviro.2016.02.002. Epub 2016 Feb 27.
Zotero 插件