Burda J, Chavko M, Gottlieb M
Physiol Bohemoslov. 1987;36(2):161-6.
Changes in the incorporation of 14C-amino acids into proteins in vitro were followed under conditions of ischemia induced by abdominal aorta ligature and subsequent recirculation in dogs. Cell saps isolated from L-S spinal cord, spinal ganglia, the sciatic nerve and medulla oblongata were added to the incorporation mixture composed of ribosomes and an enzymatic system from intact brains. Cytosols isolated from ischemic animals affected the rate of in vitro protein synthesis moderately, while repeated ischemia caused a profound decrease in the incorporation of amino acids into proteins. Cytosols from L-S spinal cord and especially from spinal ganglia after three days of recirculation substantially enhanced incorporation thus indicating a massive response of these tissues to ischemic injury. Cell saps from the medulla oblongata increased amino acid incorporation into proteins in vitro in all experimental groups.
在犬腹主动脉结扎诱导的缺血及随后的再灌注条件下，追踪了体外14C-氨基酸掺入蛋白质的变化。将从腰段脊髓、脊神经节、坐骨神经和延髓分离的细胞液加入到由核糖体和来自完整大脑的酶系统组成的掺入混合物中。从缺血动物分离的细胞溶胶适度影响体外蛋白质合成速率，而反复缺血导致氨基酸掺入蛋白质的量显著减少。再灌注三天后，来自腰段脊髓尤其是脊神经节的细胞溶胶显著增强了掺入，从而表明这些组织对缺血损伤有大量反应。在所有实验组中，延髓的细胞液增加了体外氨基酸掺入蛋白质的量。
