Chavko M, Kluchová D, Marsala J
Physiol Bohemoslov. 1981;30(3):267-74.
The authors studied the incorporation of 14C-leucine into the spinal ganglion proteins of dogs with a ligated abdominal aorta. The radioactivity of the protein fractions was studied after their electrophoretic separation in polyacrylamide gels in the presence of SDS. After 2-4 hours' ischaemia, incorporation of 14C-leucine into proteins with a molecular weight of about 100,000 fell compared with the control, while the radioactivity of proteins with a molecular weight of about 50,000 rose. The relative proportion of the protein fractions in the spinal ganglia did not alter significantly. Apart from these changes, certain qualitative changes occurred in the incidence of proteins with a molecular weight of about 40,000 during ischaemia.
作者研究了¹⁴C-亮氨酸掺入结扎腹主动脉的犬脊髓神经节蛋白质中的情况。在十二烷基硫酸钠存在下，蛋白质组分在聚丙烯酰胺凝胶中进行电泳分离后，研究其放射性。缺血2 - 4小时后，与对照组相比，¹⁴C-亮氨酸掺入分子量约为100,000的蛋白质的量减少，而分子量约为50,000的蛋白质的放射性增加。脊髓神经节中蛋白质组分的相对比例没有显著改变。除了这些变化外，缺血期间分子量约为40,000的蛋白质的发生率还发生了某些定性变化。
