Incorporation of 14C-leucine into the proteins of spinal ganglia in vivo under conditions of partial ischaemia.

The authors studied the incorporation of 14C-leucine into the spinal ganglion proteins of dogs with a ligated abdominal aorta. The radioactivity of the protein fractions was studied after their electrophoretic separation in polyacrylamide gels in the presence of SDS. After 2-4 hours' ischaemia, incorporation of 14C-leucine into proteins with a molecular weight of about 100,000 fell compared with the control, while the radioactivity of proteins with a molecular weight of about 50,000 rose. The relative proportion of the protein fractions in the spinal ganglia did not alter significantly. Apart from these changes, certain qualitative changes occurred in the incidence of proteins with a molecular weight of about 40,000 during ischaemia.