School of Resources, Environmental & Chemical Engineering and Key Laboratory of Poyang Lake Environment and Resource Utilization, Nanchang University, Nanchang, China.
Bioproducts and Biosystems Engineering Department, University of Minnesota, Saint Paul, MN 55108, United States.
Bioresour Technol. 2018 Jul;259:181-190. doi: 10.1016/j.biortech.2018.03.040. Epub 2018 Mar 10.
Fungi assisted microalgae bioflocculation is an emerging, efficient and cost-effective microalgal harvesting method, but no study has systematically evaluated and compared fungal spore-assisted (FSA) and fungal pellet-assisted (FPA) microalgal harvesting methods. In this study, harvesting Chlorella sp. cells by co-culture with Penicillium sp. spores or pellets was compared. Temperature, glucose concentration, pH and fungi:algae ratio were the critical parameters for harvesting efficiency. The highest flocculation efficiency (99%) of FSA method was achieved in 28 h at 40 °C, 160 rpm, 5 g glucose/L and 1.1 × 10 cells/mL (spore). FPA method can harvest 98.26% algae cells in 2.5 h at 34 °C, 160 rpm, pH 4.0 with the fungi:algae ratio of 1:2. The carbon input for FPA is only half of that for FSA. FPA takes less time and needs less glucose input compared with FSA and may be more promising to be further developed as an effective microalgae bioflocculation method.
真菌辅助微藻絮凝是一种新兴的、高效且经济有效的微藻收获方法，但尚无研究系统地评估和比较真菌孢子辅助（FSA）和真菌颗粒辅助（FPA）微藻收获方法。在本研究中，通过与青霉属孢子或颗粒共培养来收获小球藻细胞。温度、葡萄糖浓度、pH 值和真菌：藻类比是收获效率的关键参数。在 40°C、160rpm、5g 葡萄糖/L 和 1.1×10⁶细胞/mL（孢子）下，FSA 方法的最高絮凝效率（99%）在 28 小时内达到。在 34°C、160rpm、pH4.0 下，真菌：藻类比为 1:2 时，FPA 方法可在 2.5 小时内收获 98.26%的藻类细胞。FPA 的碳投入仅为 FSA 的一半。与 FSA 相比，FPA 所需的时间更短，葡萄糖投入更少，因此更有希望进一步开发成为有效的微藻絮凝方法。
