Chunzhuk Elizaveta A, Grigorenko Anatoly V, Kiseleva Sophia V, Chernova Nadezhda I, Ryndin Kirill G, Kumar Vinod, Vlaskin Mikhail S
Joint Institute for High Temperatures, Russian Academy of Sciences, 125412 Moscow, Russia.
Faculty of Geography, Lomonosov Moscow State University, 119991 Moscow, Russia.
Plants (Basel). 2023 Jun 28;12(13):2470. doi: 10.3390/plants12132470.
The influence of elevated CO concentrations on the growth and viability of various microalgae strains was studied. , , , , and were tested. The cultivation of microalgae was carried out at constant CO concentrations (0.04, 3, 6, or 9%-sequentially from lower to higher concentrations), under constant (24 h·day) illumination with an intensity of 74.3 µmol quanta·m·s, and a constant temperature of 23.5 ± 0.5 °C. The optical density of the microalgae biomass, pH, and the chemical composition of the culture medium were measured. Microscopy (including the cytochemical microscopic method) was conducted to monitor the state of the microalgae. The highest biomass growth rate (0.37 g·L·day), among all experiments, was achieved for at CO = 3% and for at CO = 6 and 9%. The lowest growth rate (0.12 g·L·day) was achieved for at CO = 3 and 9%. The microscopy results showed the absence or a minimum number of dead cells of the strains under selected conditions. The ability to maintain the viability of cultures up to significant concentrations of CO = 9% was due to adaptation (gradual increase in CO concentrations in the experiments).
研究了升高的CO浓度对各种微藻菌株生长和活力的影响。测试了……、……、……、……和……。微藻培养在恒定的CO浓度（0.04%、3%、6%或9%，从低浓度到高浓度依次进行）下进行，光照恒定（24小时/天），强度为74.3微摩尔光子·米²·秒²，温度恒定为23.5±0.5℃。测量了微藻生物量的光密度、pH值和培养基的化学成分。进行显微镜检查（包括细胞化学显微镜方法）以监测微藻的状态。在所有实验中，……在CO浓度为3%时以及……在CO浓度为6%和9%时实现了最高的生物量生长速率（0.37克·升⁻¹·天⁻¹）。……在CO浓度为3%和9%时实现了最低的生长速率（0.12克·升⁻¹·天⁻¹）。显微镜检查结果表明，在选定条件下，这些菌株不存在死细胞或死细胞数量最少。能够在高达9%的显著CO浓度下维持培养物的活力是由于适应（实验中CO浓度逐渐增加）。
