College of Forestry and Landscape Architecture, Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass, Key Laboratory of Energy Plants Resource and Utilization, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, and Application, Guangzhou 510640, PR China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, Guangxi 530003, PR China.
College of Forestry and Landscape Architecture, Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass, Key Laboratory of Energy Plants Resource and Utilization, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, PR China.
Bioresour Technol. 2018 Jun;258:295-301. doi: 10.1016/j.biortech.2018.03.004. Epub 2018 Mar 2.
In this work, a FeCl-catalyzed organosolv pretreatment was employed at 160 °C to remove hemicellulose and lignin in sugarcane bagasse leaving the cellulose-enriched residue for enzymatic hydrolysis to sugars. The solubilized hemicellulose fractions consisted more monomer xylose than oligomer xylose. The FeCl-catalyzed organosolv pretreatment significantly improved the enzymatic hydrolysis, nearly 100% of cellulose components were converted to glucose after pretreatment with 0.05 M FeCl. Structural analysis was employed to reveal how pretreatment affected the enzymatic hydrolysis. With the addition of Tween 80, the same level of glucose was obtained with 50% reduction of enzyme dosage after 24 h. Furthermore, the influence of Tween 80 on different pretreatment systems was investigated, indicating that the improvement was increased as the lignin content increased, decreased with high enzyme loading and extending hydrolysis time. This work suggested that the addition of Tween 80 could improve the enzymatic hydrolysis, reduce the hydrolysis time and enzyme dosage.
在这项工作中，采用 FeCl 催化的有机溶剂预处理法在 160°C 下去除甘蔗渣中的半纤维素和木质素，留下富含纤维素的残渣进行酶解转化为糖。溶解的半纤维素馏分中木糖单体的含量高于木寡糖。FeCl 催化的有机溶剂预处理显著提高了酶水解效率，用 0.05 M FeCl 预处理后，几乎 100%的纤维素成分转化为葡萄糖。结构分析揭示了预处理如何影响酶水解。添加 Tween 80 后，在 24 小时内，酶用量减少 50%，可获得相同水平的葡萄糖。此外，还研究了 Tween 80 对不同预处理体系的影响，表明随着木质素含量的增加，这种改善程度增加，随着酶用量和水解时间的延长而降低。这项工作表明，添加 Tween 80 可以提高酶水解效率，缩短水解时间和酶用量。
