College of Forestry and Landscape Architecture, Key Laboratory of Energy Plants Resource and Utilization, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research, Development, and Application, Guangzhou 510640, PR China; Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education of China, Qilu University of Technology, Jinan 250353, PR China.
College of Forestry and Landscape Architecture, Key Laboratory of Energy Plants Resource and Utilization, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, PR China.
Bioresour Technol. 2018 Feb;249:395-401. doi: 10.1016/j.biortech.2017.10.053. Epub 2017 Oct 14.
An organosolv pretreatment system consisting of 60% ethanol and 0.025 mol·L FeCl under various temperatures was developed in this study. During the pretreatment, the highest xylose yield was 11.4 g/100 g raw material, representing 49.8% of xylose in sugarcane bagasse. Structural features of raw material and pretreated substrates were characterized to better understand how hemicellulose removal and delignification affected subsequent enzymatic hydrolysis. The 160 °C pretreated solid presented a remarkable glucose yield of 93.8% for 72 h. Furthermore, the influence of different additives on the enzymatic hydrolysis of pretreated solid was investigated. The results indicated that the addition of Tween 80 shortened hydrolysis time to 6 h and allowed a 50% reduction of enzyme loading to achieve the same level of glucose yield. This work suggested that FeCl-catalyzed organosolv pretreatment could improve the enzymatic hydrolysis significantly and reduce the hydrolysis time and enzyme dosage with the addition of Tween 80.
本研究开发了一种由 60%乙醇和 0.025 mol·L FeCl 组成的有机溶剂预处理系统，在不同温度下进行。在预处理过程中，最高的木糖得率为 11.4 g/100 g 原料，相当于甘蔗渣中木糖的 49.8%。对原料和预处理底物的结构特征进行了表征，以更好地了解半纤维素去除和脱木质素如何影响后续的酶水解。160°C 预处理的固体在 72 h 内表现出显著的葡萄糖得率 93.8%。此外，还研究了不同添加剂对预处理固体酶水解的影响。结果表明，吐温 80 的添加将水解时间缩短至 6 h，并将酶用量减少 50%，即可达到相同的葡萄糖得率。这项工作表明，FeCl 催化的有机溶剂预处理可以显著提高酶水解效率，并通过添加吐温 80 缩短水解时间和减少酶用量。
Zotero 插件