College of Horticulture, Sichuan Agricultural University, Chengdu, 611130, China.
Mianyang Academy of Agricultural Sciences, Mianyang, 621023, China.
Sci Rep. 2018 Mar 19;8(1):4836. doi: 10.1038/s41598-018-23173-0.
Genetic maps are a prerequisite for quantitative trait locus (QTL) analysis, marker-assisted selection (MAS), fine gene mapping, and assembly of genome sequences. So far, several asparagus bean linkage maps have been established using various kinds of molecular markers. However, these maps were all constructed by gel- or array-based markers. No maps based on sequencing method have been reported. In this study, an NGS-based strategy, SLAF-seq, was applied to create a high-density genetic map for asparagus bean. Through SLAF library construction and Illumina sequencing of two parents and 100 F2 individuals, a total of 55,437 polymorphic SLAF markers were developed and mined for SNP markers. The map consisted of 5,225 SNP markers in 11 LGs, spanning a total distance of 1,850.81 cM, with an average distance between markers of 0.35 cM. Comparative genome analysis with four other legume species, soybean, common bean, mung bean and adzuki bean showed that asparagus bean is genetically more related to adzuki bean. The results will provide a foundation for future genomic research, such as QTL fine mapping, comparative mapping in pulses, and offer support for assembling asparagus bean genome sequence.
遗传图谱是数量性状基因座（QTL）分析、标记辅助选择（MAS）、精细基因定位和基因组序列组装的前提条件。迄今为止，已经使用各种分子标记建立了几种蕹菜连锁图谱。然而，这些图谱都是基于凝胶或基于阵列的标记构建的。目前还没有基于测序方法的图谱的报道。在本研究中，应用基于 NGS 的 SLAF-seq 策略，为蕹菜构建了一个高密度遗传图谱。通过对两个亲本和 100 个 F2 个体进行 SLAF 文库构建和 Illumina 测序，共开发和挖掘了 55,437 个多态性 SLAF 标记用于 SNP 标记。图谱由 11 个 LG 中的 5,225 个 SNP 标记组成，总长度为 1,850.81cM，标记间平均距离为 0.35cM。与其他四种豆科植物（大豆、菜豆、绿豆和赤豆）的比较基因组分析表明，蕹菜在遗传上与赤豆更为相关。研究结果将为未来的基因组研究提供基础，如 QTL 精细定位、豆类比较作图，并为组装蕹菜基因组序列提供支持。
