Chen Tianxiao, Hu Liangliang, Wang Suhua, Wang Lixia, Cheng Xuzhen, Chen Honglin
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Genet. 2022 Jul 8;13:903267. doi: 10.3389/fgene.2022.903267. eCollection 2022.
Mung bean ( L.) is an economically important grain legume cultivated in Asian countries. High-density genetic linkage is a valuable and effective tool for mapping quantitative trait loci (QTL). In the current study, a high-resolution genetic map containing 4,180 single-nucleotide polymorphisms (SNPs) was assigned to 11 linkage groups (LGs) and spanning 1,751.39 cM in length was constructed for mung bean, and the average distance between adjacent markers was 0.42 cM. Bruchids ( spp.) cause significant damage to and loss of legume seeds. A locus for bruchid resistance was detected. The gene , encoding a probable resistance-specific protein, was found to be the most likely key candidate gene in mung beans. A 69-bp sequence deletion was identified in the coding region by comparing the cDNA sequences of bruchid-resistant and bruchid-susceptible lines. This SNP-based high-density linkage map is one of the first to be constructed across the mung bean genome. This map will not only facilitate the genetic mapping of genes or complex loci that control important agronomic traits but also offer a tool for promoting future genetics and comparative genomic studies in .
绿豆（Vigna radiata (L.) R. Wilczek）是亚洲国家种植的一种具有重要经济价值的豆类作物。高密度遗传连锁图谱是定位数量性状位点（QTL）的一种有价值且有效的工具。在本研究中，构建了一张包含4180个单核苷酸多态性（SNP）的高分辨率遗传图谱，该图谱被分配到11个连锁群（LG）上，长度为1751.39厘摩（cM），相邻标记之间的平均距离为0.42厘摩。豆象（Callosobruchus spp.）会对豆类种子造成严重损害并导致损失。检测到一个抗豆象位点。发现编码一种可能的抗性特异性蛋白的基因VrNAC1是绿豆中最有可能的关键候选基因。通过比较抗豆象和感豆象品系的cDNA序列，在编码区鉴定出一个69碱基对的序列缺失。这张基于SNP的高密度连锁图谱是最早构建的跨越绿豆基因组的图谱之一。该图谱不仅将有助于控制重要农艺性状的基因或复杂位点的遗传定位，还将为促进未来绿豆的遗传学和比较基因组学研究提供一个工具。
