Scherrer Simone, Landolt Patricia, Carroli Natasha, Stephan Roger
Vetsuisse Faculty, Institute of Veterinary Bacteriology, University of Zurich, Zurich, Switzerland.
Vetsuisse Faculty, Institute for Food Safety and Hygiene, University of Zurich, Zurich, Switzerland.
Front Vet Sci. 2018 Mar 5;5:32. doi: 10.3389/fvets.2018.00032. eCollection 2018.
subsp. (MAH) is an important zoonotic pathogen with raising global health concerns. In humans, MAH is one of the most widespread non-tuberculous mycobacterial species responsible for lung disease. In animals, MAH is frequently isolated from pigs; however, it is also an opportunistic pathogen for other mammals including cattle. To elucidate the genetic diversity of MAH in cattle, a molecular characterization of isolates ( = 26) derived from lymph nodes was performed. Fourteen isolates originated from slaughtered cattle with visible altered lymph nodes at meat inspection, whereas 12 isolates were from lymph nodes without any gross pathological changes of healthy slaughtered cattle. Variable number of tandem repeat (VNTR) analysis was performed at 20 loci to examine genetic differences of isolates and to compare to previously reported VNTR data of human isolates from different countries. Genetic elements IS901, IS1245, IS1311, LSPA17, ITS1 sequevar, and hsp65 code were determined. Interestingly, two bovine MAH isolates harbored ISMav6 and hsp65 code 15, which so far has only been observed in human isolates. We supposed that VNTR data of Swiss samples would show clustering with European samples. Minimum spanning tree and unweighted pair group method using arithmetic averages analyses based on the VNTR data indicated a specific cluster of MAH isolates obtained from lymph nodes without any gross pathological changes of healthy slaughtered cattle. Comparing Swiss isolates with isolates from different other countries, no geographical clustering was observed; however, four Swiss isolates had an identical VNTR profile as human isolates from the Netherlands, the United States, and Japan. These findings indicate a possible public health issue.
亚种（MAH）是一种重要的人畜共患病原体，引发了全球日益增长的健康担忧。在人类中，MAH是导致肺部疾病的最广泛传播的非结核分枝杆菌物种之一。在动物中，MAH经常从猪身上分离出来；然而，它也是包括牛在内的其他哺乳动物的机会性病原体。为了阐明牛体内MAH的遗传多样性，对从淋巴结分离出的26株菌株进行了分子特征分析。14株菌株来自屠宰时淋巴结有明显变化的牛，而12株菌株来自健康屠宰牛且淋巴结无任何肉眼可见病理变化的样本。在20个位点进行可变数目串联重复序列（VNTR）分析，以检查菌株的遗传差异，并与先前报道的来自不同国家的人类菌株的VNTR数据进行比较。确定了遗传元件IS901、IS1245、IS1311、LSPA17、ITS1序列变体和hsp65编码。有趣的是，两株牛源MAH菌株携带ISMav6和hsp65编码15，迄今为止仅在人类菌株中观察到。我们推测瑞士样本的VNTR数据将与欧洲样本聚类。基于VNTR数据的最小生成树和算术平均法的非加权配对组分析表明，从健康屠宰牛且淋巴结无任何肉眼可见病理变化的样本中获得的MAH菌株有一个特定的聚类。将瑞士菌株与来自其他不同国家的菌株进行比较，未观察到地理聚类；然而，四株瑞士菌株与来自荷兰、美国和日本的人类菌株具有相同的VNTR图谱。这些发现表明可能存在公共卫生问题。
