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The calcium-sensitive dye arsenazo III inhibits calcium transport and ATP hydrolysis by the sarcoplasmic reticulum calcium pump.

作者信息

Riollet S, Champeil P

出版信息

Anal Biochem. 1987 Apr;162(1):160-2. doi: 10.1016/0003-2697(87)90022-4.

DOI:10.1016/0003-2697(87)90022-4
PMID:2955715
Abstract

The calcium-sensitive dye, arsenazo III, was found to interact with sarcoplasmic reticulum vesicles and to inhibit markedly the rate of calcium transport and ATP hydrolysis by these vesicles, thus perturbing the transport process it was meant to monitor. Caution should therefore be exercised when using this dye to monitor calcium movements.

摘要

相似文献

1
The calcium-sensitive dye arsenazo III inhibits calcium transport and ATP hydrolysis by the sarcoplasmic reticulum calcium pump.
Anal Biochem. 1987 Apr;162(1):160-2. doi: 10.1016/0003-2697(87)90022-4.
2
Oxidation of sulfhydryl groups and inhibition of the (Ca2+ + Mg2+)-ATPase by arsenazo III.
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3
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Biochem Soc Trans. 1979 Oct;7(5):1101-2. doi: 10.1042/bst0071101.
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Determination of coupling ratios of the calcium pump of sarcoplasmic reticulum by pulse methods.用脉冲法测定肌浆网钙泵的偶联比
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5
The rate of Ca2+ translocation by sarcoplasmic reticulum (Ca2+ + Mg2+)-ATPase measured with intravesicular arsenazo III.
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Vanadate oligomer inhibition of passive and active Ca2+ translocation by the Ca2+ pump of sarcoplasmic reticulum.钒酸盐低聚物对肌浆网钙泵被动和主动转运Ca2+的抑制作用。
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Interaction of metallochromic indicators with calcium sequestering organelles.
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Steady state kinetics of ATP synthesis and hydrolysis coupled calcium transport catalyzed by the reconstituted sarcoplasmic reticulum ATPase.重构肌浆网ATP酶催化的ATP合成与水解偶联钙转运的稳态动力学
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Effects of phenothiazine drugs on the active Ca2+ transport by sarcoplasmic reticulum.吩噻嗪类药物对肌浆网主动钙转运的影响。
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