Baquey C, Sigot-Luizard M F, Friede J, Prud'hom R E, Guidoin R G
Biomaterials. 1987 May;8(3):185-9. doi: 10.1016/0142-9612(87)90061-5.
The study reported here is concerned with the radio-sterilization of Dacron vascular prostheses coated with crosslinked albumin. gamma-Radiations have no effect on the mechanical properties of the polyester fibres or on their crystallinity, whether irradiated in a dry state or immersed in saline. Special attention has been paid to the release of the albumin, or protein fragments from the reticulum using 125I-labelled albumin as a radiotracer. The albumin leakage depends upon the type of Dacron fabrics considered but the values derived from radioactivity measurements are always greater than those directly measured, which indicates a radio-induced break of the bond between iodine and albumin; this has nothing to do with the break of the association between albumin and Dacron. Moreover no cytotoxicity of the irradiated immersion medium has been observed using a test based on organotypic culture in liquid medium. Thus radio-sterilization of an albuminated polyester vascular prosthesis immersed in saline appears to be a suitable procedure.
本文报道的研究涉及涂有交联白蛋白的涤纶血管移植物的辐射灭菌。无论在干燥状态下还是浸入盐水中进行辐照，γ射线对聚酯纤维的机械性能或结晶度均无影响。我们特别关注使用125I标记的白蛋白作为放射性示踪剂时，白蛋白或蛋白质片段从网状结构中的释放情况。白蛋白泄漏取决于所考虑的涤纶织物类型，但放射性测量得出的值总是大于直接测量的值，这表明存在辐射诱导的碘与白蛋白之间化学键的断裂；这与白蛋白和涤纶之间结合的断裂无关。此外，使用基于液体培养基中器官型培养的测试，未观察到辐照后的浸泡介质具有细胞毒性。因此，浸入盐水中的白蛋白化聚酯血管移植物的辐射灭菌似乎是一种合适的方法。
