Franke W W, Kapprell H P, Cowin P
Biol Cell. 1987;59(3):205-18. doi: 10.1111/j.1768-322x.1987.tb00532.x.
We have characterized the junctions between endothelial cells of diverse blood vessels at the light and electron microscopic level using various antibodies to plakoglobin (polypeptide Mr 83,000) and vinculin. Endothelial cells from fenestrated and non-fenestrated capillaries to large arteries are connected to each other by extended junctions that are coated on their cytoplasmic face by plaques of loosely matted filamentous material that form a continuous belt system along the cell circumference. These plaques are devoid of desmosome-specific proteins such as desmoplakin(s) and desmoglein, but contain plakoglobin. Immunofluorescence microscopic reactions of these regions with vinculin antibodies have also been observed, although they are much weaker and less consistent. This composition, together with their association with actin microfilaments, classifies this extended plaque system as Zonulae adhaerentes. Our results also show that such endothelia may be distinguished from truly epithelial cells by the absence of desmosomes and intermediate filaments of the cytokeratin type. The relationship of the various kinds of adhering junctions and the physiological importance of these junctions are discussed.
我们利用多种针对桥粒斑珠蛋白（分子量83,000的多肽）和纽蛋白的抗体，在光学显微镜和电子显微镜水平上对不同血管内皮细胞之间的连接进行了表征。从有孔和无孔毛细血管到大动脉的内皮细胞通过延伸连接相互连接，这些连接在其细胞质面被松散交织的丝状物质斑块覆盖，这些斑块沿着细胞周长形成连续的带状系统。这些斑块缺乏桥粒特异性蛋白，如桥粒斑蛋白和桥粒芯糖蛋白，但含有桥粒斑珠蛋白。尽管这些区域与纽蛋白抗体的免疫荧光显微镜反应较弱且不太一致，但也已观察到。这种组成，连同它们与肌动蛋白微丝的关联，将这种延伸的斑块系统归类为黏着小带。我们的结果还表明，这种内皮细胞可能与真正的上皮细胞不同，因为它们没有桥粒和细胞角蛋白类型的中间丝。讨论了各种黏着连接的关系以及这些连接的生理重要性。
