State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Mar Drugs. 2018 Mar 20;16(3):98. doi: 10.3390/md16030098.
Mycemycins A-E are new members of the dibenzoxazepinone (DBP) family, derived from the gene-disrupted deep sea strain FXJ8.012Δ1741 and the soil strain sp. FXJ1.235. In this paper, we report the identification of the gene clusters and pathways' inference for mycemycin biosynthesis in the two strains. Bioinformatics analyses of the genome sequences of FXJ8.012Δ1741 and . sp. FXJ1.235 predicted two divergent mycemycin gene clusters, and , respectively. Heterologous expression of the key enzyme genes of and genetic manipulation of as well as a feeding study in . sp. FXJ1.235 confirmed the gene clusters and led to the proposed biosynthetic pathways for mycemycins. To the best of our knowledge, this is the first report on DBP biosynthetic gene clusters and pathways.
我的霉素 A-E 是来自基因缺失深海菌株 FXJ8.012Δ1741 和土壤菌株 sp. FXJ1.235 的二苯并恶嗪酮 (DBP) 家族的新成员。在本文中，我们报告了在这两个菌株中鉴定出的 mycemycin 生物合成基因簇和途径推断。FXJ8.012Δ1741 和 sp. FXJ1.235 的基因组序列的生物信息学分析分别预测了两个发散的 mycemycin 基因簇和。异源表达和遗传操作以及 sp. FXJ1.235 的喂养研究证实了基因簇，并提出了 mycemycins 的生物合成途径。据我们所知，这是 DBP 生物合成基因簇和途径的第一个报告。
Zotero 插件
