Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali, University of Messina, Via Consolare Valeria, 98125, Messina, Italy.
Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Polo Annunziata, viale Annunziata, 98168, Messina, Italy.
Arch Biochem Biophys. 2018 May 15;646:161-167. doi: 10.1016/j.abb.2018.03.011. Epub 2018 Mar 17.
An on-line method based on the coupling of supercritical fluid extraction and supercritical fluid chromatography with triple quadrupole mass spectrometry detection (SFE-SFC-QqQ/MS) for the native carotenoids characterization and apocarotenoids detection in yellow tamarillo was developed for the first time; this is the first work reporting the application of this methodology for the apocarotenoids analysis in any matrix. An off-line liquid extraction (LE) and analysis by LC-PDA-MS, using both a conventional C30 (3 μm I.D. particles) and a novel C30 column with sub-2 micron particles were also performed. 31 compounds were extracted and identified by the developed SFE-SFC-QqQ/MS methodology in less than 18 min, including free carotenoids, carotenoids monoesters, carotenoids diesters and apocarotenoids in a very fast, and efficient way; moreover among those 31 compounds, 3 antheraxanthin monoesters and 9 apocarotenoids were detected in yellow tamarillo for the first time, namely apo-8'-zeaxanthinal, apo-10'-zeaxanthinal, apo-12'-zeaxanthinal, apo-14'-zeaxanthinal, apo-15'-zeaxanthinal, apo-12'-carotenal, apo-14'-carotenal and the two apocarotenoids esters apo-10'-zeaxanthinal-C4:0 and apo-8'-zeaxanthinal-C12:0. Further, the novel sub-2 micron particles C30 column, showed a better performance compared to the conventional C30 one. Finally a quantitative comparison of two selected carotenoids was performed by using SFE-SFC-QqQ/MS, SFC-QqQ/MS, and LC-PDA, which showed overall comparable results.
首次开发了一种基于超临界流体萃取和超临界流体色谱与三重四极杆质谱联用（SFE-SFC-QqQ/MS）的在线方法，用于对黄色番荔枝中天然类胡萝卜素的特征描述和类胡萝卜素降解产物的检测；这是首次报道该方法在任何基质中分析类胡萝卜素降解产物的应用。还进行了离线液提取（LE）和通过 LC-PDA-MS 的分析，同时使用了常规的 C30（3 µm I.D. 颗粒）和具有亚 2 微米颗粒的新型 C30 柱。通过开发的 SFE-SFC-QqQ/MS 方法，在不到 18 分钟的时间内提取并鉴定了 31 种化合物，包括游离类胡萝卜素、类胡萝卜素单酯、类胡萝卜素二酯和类胡萝卜素降解产物，方法快速且高效；此外，在这 31 种化合物中，首次在黄色番荔枝中检测到 3 种花药黄质单酯和 9 种类胡萝卜素降解产物，即脱-8'-玉米黄质、脱-10'-玉米黄质、脱-12'-玉米黄质、脱-14'-玉米黄质、脱-15'-玉米黄质、脱-12'-胡萝卜素醛、脱-14'-胡萝卜素醛和两种类胡萝卜素降解产物酯脱-10'-玉米黄质-C4：0 和脱-8'-玉米黄质-C12：0。此外，新型的亚 2 微米颗粒 C30 柱与常规 C30 柱相比表现出更好的性能。最后，通过 SFE-SFC-QqQ/MS、SFC-QqQ/MS 和 LC-PDA 对两种选定的类胡萝卜素进行了定量比较，结果总体上具有可比性。
