Lu Jianyun, Li Miaomiao, Gao Lihua, Pan Yizhi, Xiang Yaping, Huang Jinhua, Jiang Xianzhen
Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Department of Urology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2018 Feb 28;43(2):143-146. doi: 10.11817/j.issn.1672-7347.2018.02.006.
To determine initial concentrations of ozonated water under different temperatures, attenuation rules of ozonated water under the room temperature (25 ℃), and to inspect the effects of ozonated water under different concentrations on common microorganisms. Methods: The online test method and the plate cultivation method were employed to check the concentrations and killing rates on common microorganisms of ozonated water produced by HZ-2601 B Ozone Water Generating Instrument. Results: The initial concentrations of ozonated water at 20, 25, 30, 35, and 40 ℃ were 4.38, 4.26, 3.12, 2.76, and 1.31 mg/L, respectively. The ozonated water was rapidly attenuated at first 10 min. The concentration of ozonated water still remained at 1.06 mg/L and 0.37 mg/L at 25 and 30 ℃ after 30 min. The average killing rates for Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and Candida albicans in 1.0 mg/L ozonated water for 1 min were 99%, 100%, 100%, 100%, and 100%, respectively. The average killing rates of Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans in 0.3 mg/L ozonated water for 1 min were 100%, 100%, 100%, 95%, and 92%, respectively. Conclusion: The initial concentrations of ozonated water produced by HZ-2601 B Ozone Water Generating Instrument decrease with the increase of temperature. Ozonated water under 20-30 ℃ has good sterilization effect on common microorganisms.
测定不同温度下臭氧水的初始浓度、室温（25℃）下臭氧水的衰减规律，并考察不同浓度臭氧水对常见微生物的作用。方法：采用在线检测法和平板培养法检测HZ-2601B型臭氧水发生器产生的臭氧水对常见微生物的浓度及杀灭率。结果：20、25、30、35和40℃时臭氧水的初始浓度分别为4.38、4.26、3.12、2.76和1.31mg/L。臭氧水在最初10min迅速衰减。30min后，25℃和30℃时臭氧水浓度分别仍为1.06mg/L和0.37mg/L。1.0mg/L臭氧水中，铜绿假单胞菌、大肠埃希菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和白色念珠菌作用1min的平均杀灭率分别为99%、100%、100%、100%和100%。0.3mg/L臭氧水中，大肠埃希菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌作用1min的平均杀灭率分别为100%、100%、100%、95%和92%。结论：HZ-2601B型臭氧水发生器产生的臭氧水初始浓度随温度升高而降低。20 - 30℃的臭氧水对常见微生物有良好的杀菌效果。
