Sinha Anuradha, Kanungo Suman, Kim Deok Ryun, Manna Byomkesh, Song Manki, Park Ju Yeon, Haldar Bisakha, Sharma Prashant, Mallick Aiyel Haque, Kim Soon Ae, Babji Sudhir, Sur Dipika, Kang Gagandeep, Ali Mohammad, Petri William A, Wierzba Thomas F, Czerkinsky Cecil, Nandy Ranjan Kumar, Dey Ayan
National Institute of Cholera and Enteric Diseases, Kolkata, India.
International Vaccine Institute, Seoul, South Korea.
Heliyon. 2018 Feb 1;4(1):e00519. doi: 10.1016/j.heliyon.2018.e00519. eCollection 2018 Jan.
Assessing immune response after rotavirus vaccination consists in measuring serum or plasma IgA and IgG antibodies, but these assays provide very little information about the mucosal immune response. Thus the development of assays for detection of mucosal immune response following rotavirus vaccination is essential. We evaluate to assess circulating antibody-secreting cells (ASCs) as a potential means to evaluate mucosal immune responses to rotavirus vaccine.
372 subjects, aged 6 weeks, were enrolled in the study. All the subjects were assigned to receive two doses of Rotarix vaccine. Using a micro-modified whole blood-based ELISPOT assay, circulating rotavirus type-specific IgA- and IgG-ASCs, including gut homing β7+ ASCs, were enumerated on week 6 before the first dose of Rotarix vaccination at 7 weeks of age and week 18 after the second vaccination at 17 weeks of age. Plasma samples collected before vaccination, and after two doses of Rotarix vaccination were tested for plasma rotavirus IgA titers.
Two doses of Rotarix provided to induce sero-protective titer of ≥ 20 Units in 35% of subjects. Total blood IgA- ASC responses were detected in 26.4% of subjects who were non-responder before vaccination. Among responders, 47% of the subjects also have sero-protective plasma IgA titers.
Our results suggest that virus-specific blood gut homing ASCs were detected and provide insight into mucosal immune response after rotavirus vaccination. Further studies are needed to evaluate the duration of such immune responses and to assess the programmatic utility of this whole blood-based mucosal ASC testing for the rotavirus immunization program.
评估轮状病毒疫苗接种后的免疫反应包括测量血清或血浆中的IgA和IgG抗体，但这些检测方法提供的关于黏膜免疫反应的信息非常少。因此，开发用于检测轮状病毒疫苗接种后黏膜免疫反应的检测方法至关重要。我们评估将循环抗体分泌细胞（ASC）作为评估轮状病毒疫苗黏膜免疫反应的一种潜在手段。
372名6周龄的受试者参与了该研究。所有受试者均被分配接受两剂Rotarix疫苗。使用微改良的基于全血的ELISPOT检测方法，在7周龄首次接种Rotarix疫苗前6周以及17周龄第二次接种后18周，对循环中的轮状病毒特异性IgA和IgG-ASC，包括肠道归巢β7+ ASC进行计数。在接种疫苗前以及两剂Rotarix疫苗接种后采集的血浆样本，检测血浆轮状病毒IgA滴度。
两剂Rotarix疫苗使35%的受试者诱导出≥20单位的血清保护滴度。在接种前无反应的受试者中，26.4%检测到全血IgA-ASC反应。在有反应者中，47%的受试者也具有血清保护血浆IgA滴度。
我们的结果表明，检测到了病毒特异性的血液肠道归巢ASC，并为轮状病毒疫苗接种后的黏膜免疫反应提供了见解。需要进一步研究来评估这种免疫反应的持续时间，并评估这种基于全血的黏膜ASC检测在轮状病毒免疫规划中的实用性。
