Cordeiro Rossana de Aguiar, Weslley Caracas Cedro Ewerton, Raquel Colares Andrade Ana, Serpa Rosana, José de Jesus Evangelista Antonio, Sales de Oliveira Jonathas, Santos Pereira Vandbergue, Pereira Alencar Lucas, Bruna Leite Mendes Patrícia, Cibelle Soares Farias Bárbara, Maria Maciel Melo Vânia, Pires de Camargo Zoilo, de Souza Collares Maia Castelo-Branco Débora, Sâmia Nogueira Brilhante Raimunda, Júlio Costa Sidrim José, Fábio Gadelha Rocha Marcos
a Department of Pathology and Legal Medicine , Federal University of Ceará , Fortaleza , Brazil.
b Department of Pathology and Legal Medicine , Federal University of Santa Catarina , Fortaleza , Brazil.
Biofouling. 2018 Mar;34(3):309-319. doi: 10.1080/08927014.2018.1437617. Epub 2018 Mar 21.
The present study aimed to investigate the inhibitory effect of a bacterial biosurfactant (TIM96) on clinical strains of Trichosporon. Additionally, the effect of TIM96 on the ergosterol content, cell membrane integrity, and the hydrophobicity of planktonic cells was assessed. The inhibitory activity of TIM96 against Trichosporon biofilms was evaluated by analyzing metabolic activity, biomass and morphology. MIC values ranged from 78.125 to 312.5 μg ml for TIM96; time-kill curves revealed that the decline in the number of fungal cells started after incubation for 6 h with TIM96 at both MIC and 2×MIC. The biosurfactant reduced the cellular ergosterol content and altered the membrane permeability and the surface hydrophobicity of planktonic cells. Incubation at 10×MIC TIM96 reduced cell adhesion by up to 96.89%, thus interfering with biofilm formation. This concentration also caused up to a 99.2% reduction in the metabolic activity of mature biofilms. The results indicate potential perspectives for the development of new antifungal strategies.
本研究旨在探讨一种细菌生物表面活性剂（TIM96）对临床曲霉菌株的抑制作用。此外，还评估了TIM96对麦角固醇含量、细胞膜完整性以及浮游细胞疏水性的影响。通过分析代谢活性、生物量和形态来评估TIM96对曲霉菌生物膜的抑制活性。TIM96的最低抑菌浓度（MIC）值范围为78.125至312.5μg/ml；时间杀菌曲线显示，在MIC和2×MIC浓度下，与TIM96孵育6小时后，真菌细胞数量开始下降。该生物表面活性剂降低了细胞麦角固醇含量，并改变了浮游细胞的膜通透性和表面疏水性。在10×MIC的TIM96浓度下孵育可使细胞粘附减少高达96.89%，从而干扰生物膜形成。该浓度还可使成熟生物膜的代谢活性降低高达99.2%。结果表明开发新的抗真菌策略具有潜在前景。
