Wang Qian, Song Yali, Choi Lina, Liu Hongyu, Wang Gejiao, Li Mingshun
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Int J Syst Evol Microbiol. 2018 May;68(5):1622-1626. doi: 10.1099/ijsem.0.002724. Epub 2018 Mar 21.
A Gram-stain-negative, non-motile, rod-shaped, red-pigmented strain, designated W37, was isolated from soil near an iron factory in Busan (Republic of Korea). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain W37 was most closely related to Deinococcus yunweiensis YIM 007 (98.3 %) and Deinococcus radioresistens 8A (96.3 %). The DNA-DNA relatedness between strain W37 and D. yunweiensis YIM 007 was 50.5 %. The predominant respiratory quinone was MK-8. The major polar lipids were an unidentified phosphoglycolipid, an unidentified aminophospholipid, four unidentified glycolipids, two unidentified phospholipids and an unidentified lipid. The major fatty acids (>5 %) of strain W37 were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0, C17 : 1ω8c and iso-C17 : 1ω9c. The DNA G+C content was 69.0 mol%. Moreover, the chemo-physical characteristics of strain W37 clearly differed from those of related species, including ranges of growth temperature and pH, positive activity for 4-hydroxybenzoate and negative activity for cystine arylamidase. Phenotypic, chemotaxonomic and genotypic analyses indicated that strain W37 represents a novel species of the genus Deinococcus, for which the name Deinococcus rufus sp. nov., is proposed. The type strain is W37 (=KCTC 33913=CCTCC AB 2017081).
从韩国釜山一家铁厂附近的土壤中分离出一株革兰氏染色阴性、无运动性、杆状、产红色色素的菌株，命名为W37。基于16S rRNA基因序列的系统发育分析表明，菌株W37与云南嗜辐射球菌YIM 007（98.3%）和耐辐射嗜辐射球菌8A（96.3%）关系最为密切。菌株W37与云南嗜辐射球菌YIM 007之间的DNA-DNA相关性为50.5%。主要呼吸醌为MK-8。主要极性脂类为一种未鉴定的磷酸糖脂、一种未鉴定的氨基磷脂、四种未鉴定的糖脂、两种未鉴定的磷脂和一种未鉴定的脂类。菌株W37的主要脂肪酸（>5%）为总和特征3（C16:1ω7c和/或异-C15:0 2-OH）、C16:0、C17:1ω8c和异-C17:1ω9c。DNA G+C含量为69.0 mol%。此外，菌株W37的化学物理特性与相关物种明显不同，包括生长温度和pH范围、对4-羟基苯甲酸的阳性活性和对胱氨酸芳基酰胺酶的阴性活性。表型、化学分类学和基因型分析表明，菌株W37代表嗜辐射球菌属的一个新物种，为此提出名为嗜辐射红球菌（Deinococcus rufus sp. nov.）。模式菌株为W37（=KCTC 33913=CCTCC AB 2017081）。
