Maisch B, Izumi T
Heart Vessels Suppl. 1985;1:8-13. doi: 10.1007/BF02072351.
New aspects in the research of myocarditis and dilated cardiomyopathy in West Germany have evolved from molecular biology, immunobiology of the mitochondrion, immunoserology, and immunohistology. Coxsackie B3 virus inoculated into fetal human myocytes induced myocytolysis in the absence of immunologic effector mechanisms. By pretreatment with beta-interferon, the virus yield from the myocytes was reduced significantly. In myocarditis and dilated cardiomyopathy, antibodies against an organ-specific autoantigen of cardiac mitochondria, the adenine nucleotide translocator, were demonstrated. Antibody titers roughly correlated with the ejection fraction using the Elisa technique. It could also be shown that in 13% of cases in myocarditis and 31% in dilated cardiomyopathy heart-associated antimitochondrial antibodies are found, called anti-M7. Most of the patients had an interfibrillary staining pattern in the immunofluorescence test. No correlation with the severity of heart disease could be established. In dilated cardiomyopathy and myocarditis, there has recently been controversy over low suppressor T-cell activity. Whereas other groups have demonstrated a low concanavaldin-A-induced suppressor T-cell activity in both diseases, we have not been able to confirm reduced Con-A-induced or spontaneous T-suppressor cell activity in the different indicator systems used in analysis.
西德心肌炎和扩张型心肌病研究的新进展源自分子生物学、线粒体免疫生物学、免疫血清学及免疫组织学。将柯萨奇B3病毒接种到胎儿人类心肌细胞中，在无免疫效应机制的情况下可诱导心肌细胞溶解。通过用β干扰素预处理，心肌细胞中的病毒产量显著降低。在心肌炎和扩张型心肌病中，已证实存在针对心脏线粒体一种器官特异性自身抗原——腺嘌呤核苷酸转位酶的抗体。使用酶联免疫吸附测定技术，抗体滴度大致与射血分数相关。还可表明，在13%的心肌炎病例和31%的扩张型心肌病病例中发现了心脏相关抗线粒体抗体，称为抗M7。大多数患者在免疫荧光试验中呈现肌原纤维间染色模式。无法确定其与心脏病严重程度的相关性。在扩张型心肌病和心肌炎中，最近对于低抑制性T细胞活性存在争议。虽然其他研究小组已证明这两种疾病中伴刀豆球蛋白A诱导的抑制性T细胞活性较低，但我们未能在分析中使用的不同指标系统中证实伴刀豆球蛋白A诱导的或自发的T抑制细胞活性降低。
