兔体内自身抗体的产生。II. 注射大鼠组织的兔体内的器官特异性自身抗体。
Autoantibody production in rabbits. II. Organ-specific autontibody in rabbits injected with rat tissues.
作者信息
ASHERSON G L, DUMONDE D C
出版信息
Immunology. 1963 Jan;6(1):19-29.
The sera of rabbits injected with rat liver, kidney, heart, muscle, spleen and brain in Freund's complete adjuvant fixed complement with rabbit tissue. This complement-fixing activity was attributed to autoantibodies which were able to fix complement with the tissue of the rabbit in which they occurred. Absorption, gel diffusion and antibody and antigen titrations indicated that some of the anti-liver, anti-kidney, anti-heart, anti-muscle and anti-brain sera contained organ-specific autoantibody. The sera also contained autoantibody reacting with widely distributed antigen(s), which was relatively labile at 65°. The anti-kidney and anti-brain sera reacted with distinct antigens which were extracted from rabbit kidney and brain with a mixture of chloroform and methanol. The natural autoantibody of Kidd and Friedewald was usually labile at 65° and behaved like a macroglobulin on sucrose gradient centrifugation. Sera taken 1 week after immunization with rat tissue contained heat-labile macroglobulin antibody. However, sera taken 1 month after immunization also contained small molecular weight antibody which was stable at 65°.
用弗氏完全佐剂注射大鼠肝脏、肾脏、心脏、肌肉、脾脏和脑的兔血清，能与兔组织固定补体。这种补体固定活性归因于自身抗体，这些自身抗体能与产生它们的兔组织固定补体。吸收试验、凝胶扩散试验以及抗体和抗原滴定表明，一些抗肝脏、抗肾脏、抗心脏、抗肌肉和抗脑血清含有器官特异性自身抗体。这些血清还含有与广泛分布的抗原发生反应的自身抗体，该抗原在65°时相对不稳定。抗肾脏和抗脑血清与用氯仿和甲醇混合物从兔肾脏和脑中提取的不同抗原发生反应。基德和弗里德瓦尔德的天然自身抗体通常在65°时不稳定，在蔗糖梯度离心中表现得像一种巨球蛋白。用大鼠组织免疫1周后采集的血清含有热不稳定的巨球蛋白抗体。然而，免疫1个月后采集的血清也含有在65°时稳定的小分子抗体。
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