Robles L J, Watanabe A, Kremer N E, Wong F, Bok D
J Neurocytol. 1987 Jun;16(3):403-15. doi: 10.1007/BF01611350.
The photopigments, rhodopsin and retinochrome, have been localized in cephalopod retinae using light and electron microscopic immunocytochemical methods. Polyclonal antibodies prepared against squid opsin demonstrated the presence of this protein in the photoreceptor rhabdomes, Golgi zone, Golgi-associated vesicles, plasma membrane, large cytoplasmic vesicles, and axonal membranes of octopus retinae. Monoclonal anti-opsin immunostained the rhabdomes and multivesicular bodies in the photoreceptor inner segments of squid. We believe the multivesicular bodies are involved in rhabdome turnover. Polyclonal anti-retinochrome localized this photopigment to the myeloid bodies of the photoreceptor inner segments, the rhabdomes, and to the extracellular space between opposing rhabdomeres in octopus retina. The results suggest some interesting functional relationships between rhodopsin and retinochrome with regard to chromophore exchange between illuminated forms of these photopigments and chromophore addition to newly synthesized opsin.
利用光学显微镜和电子显微镜免疫细胞化学方法，已将视色素、视紫红质和视网膜视色素定位到头足类动物的视网膜中。针对鱿鱼视蛋白制备的多克隆抗体表明，这种蛋白质存在于章鱼视网膜的光感受器视杆、高尔基体区、高尔基体相关小泡、质膜、大的细胞质小泡和轴突膜中。单克隆抗视蛋白免疫染色了鱿鱼光感受器内段的视杆和多囊体。我们认为多囊体参与视杆的更新。多克隆抗视网膜视色素将这种视色素定位到章鱼视网膜光感受器内段的髓样体、视杆以及相对视小杆之间的细胞外空间。结果表明，视紫红质和视网膜视色素在这些视色素的光照形式之间的发色团交换以及新合成视蛋白的发色团添加方面存在一些有趣的功能关系。
