Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.
Department of Plastic and Aesthetic Surgery, University Hospital Olomouc, I. P. Pavlova 6, 779 00, Olomouc, Czech Republic.
Arch Dermatol Res. 2018 Jul;310(5):413-424. doi: 10.1007/s00403-018-1828-6. Epub 2018 Mar 21.
Exposure to solar radiation is a major cause of environmental human skin damage. The main constituent of solar UV light is UVA radiation (320-400 nm); however, the need for protection against UVA has been marginalized for a long time. As a result, there is still a lack of useful agents for UVA protection. In this study, the effect of silymarin (SM) and its main constituent silybin (SB) pre-treatment on UVA-stimulated damage to primary human dermal fibroblasts were carried out. The cells were pre-treated for 1 h with SB or SM and then were exposed to UVA light, using a solar simulator. The effect of SB and SM on reactive oxygen species (ROS) and glutathione (GSH) level, caspase-3 activity, single-strand breaks (SSB) formation and protein level of matrix metalloproteinase-1 (MMP-1), heme oxygenase-1 (HO-1), and heat shock protein (HSP70) was evaluated. Treatment with both SM and SB resulted in a reduction in UVA-stimulated ROS generation and SSB production, as well as in the prevention of GSH depletion, a decrease in the activation of caspase-3 and protein level of MMP-1. They also moderately increased HO-1 level and reduced HSP70 level. Our data showed that both SM and SB are non-phototoxic and have UVA-photoprotective potential and could be useful agents for UV-protective dermatological preparations.
暴露于太阳辐射是造成环境性人体皮肤损伤的主要原因。太阳紫外线的主要成分是 UVA 辐射（320-400nm）；然而，长期以来，人们一直忽视了对 UVA 的防护需求。因此，仍然缺乏有效的 UVA 防护剂。在这项研究中，我们进行了水飞蓟素（SM）及其主要成分水飞蓟宾（SB）预处理对人原代真皮成纤维细胞 UVA 刺激损伤的影响。用太阳模拟器对细胞进行 1 小时的 SB 或 SM 预处理，然后暴露于 UVA 光下。评估了 SB 和 SM 对活性氧（ROS）和谷胱甘肽（GSH）水平、半胱天冬酶-3 活性、单链断裂（SSB）形成以及基质金属蛋白酶-1（MMP-1）、血红素加氧酶-1（HO-1）和热休克蛋白（HSP70）蛋白水平的影响。SM 和 SB 的处理均可减少 UVA 刺激的 ROS 生成和 SSB 产生，并防止 GSH 耗竭、半胱天冬酶-3 激活和 MMP-1 蛋白水平降低。它们还适度增加了 HO-1 水平并降低了 HSP70 水平。我们的数据表明，SM 和 SB 均无光毒性，具有 UVA 光保护潜力，可作为用于防晒护肤制剂的有用试剂。
