Arzate-Mejía Rodrigo G, Licona-Limón Paula, Recillas-Targa Félix
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, México.
Departamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, México.
Methods Mol Biol. 2018;1752:177-190. doi: 10.1007/978-1-4939-7714-7_16.
Over the years, the study of gene function during development involved the implementation of sophisticated transgenic strategies to visualize how organisms change during their lifetime. These strategies are diverse and extremely useful and allowed the discovery of some of the fundamental mechanisms governing organism's development. Such strategies can be time-consuming, in some cases expensive, and require complex infrastructure. With the advent of the genome editing CRISPR-Cas9 RNA-guided DNA endonuclease system a tremendous progress has been achieved in manipulating diverse organisms and cell types. In recent years this system has contributed importantly to the design of novel experimental strategies to further understand developmental processes, to generate genetically modified animal models, and develop disease models. Here we highlight examples in which the genome editing CRISPR-Cas9 system has been employed to understand the mechanisms controlling embryonic development and disease.
多年来，发育过程中基因功能的研究涉及实施复杂的转基因策略，以观察生物体在其生命周期中的变化。这些策略多种多样且极为有用，使得一些控制生物体发育的基本机制得以发现。此类策略可能耗时，在某些情况下成本高昂，并且需要复杂的基础设施。随着基因组编辑CRISPR-Cas9 RNA引导的DNA内切核酸酶系统的出现，在操纵各种生物体和细胞类型方面取得了巨大进展。近年来，该系统对设计新的实验策略以进一步理解发育过程、生成转基因动物模型以及开发疾病模型做出了重要贡献。在此，我们重点介绍基因组编辑CRISPR-Cas9系统被用于理解控制胚胎发育和疾病机制的实例。
