视网膜变性啮齿动物的神经节细胞评估

Ganglion Cell Assessment in Rodents with Retinal Degeneration.

作者信息

Fletcher Erica L, Greferath Ursula, Saha Susmita, Anderson Emily E, Vessey Kirstan A

机构信息

Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne, VIC, Australia.

出版信息

Methods Mol Biol. 2018;1753:261-273. doi: 10.1007/978-1-4939-7720-8_18.

DOI:10.1007/978-1-4939-7720-8_18

PMID:29564795

Abstract

Analysis of how retinal ganglion cells change in retinal degeneration is critical for evaluating the potential of photoreceptor restorative therapies. Immunocytochemistry in combination with image analysis provides a way for quantifying not only the density of ganglion cells during disease, but also information about their morphology and an evaluation of excitatory and inhibitory synaptic inputs. Here, we describe how indirect immunofluorescence can be used in retinal whole mounts to obtain information about ganglion cells in retinal degeneration.

摘要

分析视网膜神经节细胞在视网膜变性过程中的变化对于评估光感受器修复疗法的潜力至关重要。免疫细胞化学与图像分析相结合，不仅为量化疾病期间神经节细胞的密度提供了一种方法，还能提供有关其形态的信息以及对兴奋性和抑制性突触输入的评估。在此，我们描述了如何在视网膜全层标本中使用间接免疫荧光来获取有关视网膜变性中神经节细胞的信息。