肥胖型哮喘中 Muc5ac 高分泌与 Munc18b 上调的关系。
The relationship between Muc5ac high secretion and Munc18b upregulation in obese asthma.
机构信息
Department of Respiratory, Qingdao University, Qingdao Municipal Hospital, Qingdao, Shandong, China.
出版信息
Eur Rev Med Pharmacol Sci. 2018 Mar;22(5):1409-1414. doi: 10.26355/eurrev_201803_14487.
OBJECTIVE
Mucus production and hypersecretion are important pathophysiological features of asthma. Airway mucus secretion is more serious in obese asthma. Therefore, it is of great significance to elucidate the mechanism of asthma airway mucus high secretion in improving the control of asthma and the prognosis of obese asthmatic patients.
MATERIALS AND METHODS
Obese asthmatic mice model was established to test the airway resistance and mucin secretion by hematoxylin-eosin (HE) staining. Munc18b and Muc5ac expression levels were determined by Western-blotting. Munc18b conditioned knockout mice were adopted to explore the mechanism of Muc5ac high secretion.
RESULTS
The mice weight increased in obese asthmatic model accompanied by elevated airway resistance. HE staining showed enhanced mucin secretion, which was correlated to weight and airway resistance. Munc18b and Muc5ac expressions significant upregulated in an obese asthmatic mouse model compared with normal control. Muc5ac expression failed to show elevation in Munc18b conditioned knockout mice.
CONCLUSIONS
Muc5ac high secretion was positively correlated with Munc18b upregulation in obese asthma. Munc18b participated in inducing Muc5ac high expression.
目的
黏液产生和分泌亢进是哮喘的重要病理生理特征。肥胖型哮喘患者的气道黏液分泌更为严重。因此，阐明哮喘气道黏液高分泌的机制，对于改善哮喘控制和肥胖型哮喘患者的预后具有重要意义。
材料与方法
通过苏木精-伊红（HE）染色检测气道阻力和黏蛋白分泌，建立肥胖型哮喘小鼠模型。采用 Western-blotting 检测 Munc18b 和 Muc5ac 的表达水平。采用 Munc18b 条件性敲除小鼠探索 Muc5ac 高分泌的机制。
结果
肥胖型哮喘模型中，小鼠体重增加，同时气道阻力升高。HE 染色显示黏蛋白分泌增强，与体重和气道阻力相关。与正常对照组相比，肥胖型哮喘小鼠模型中 Munc18b 和 Muc5ac 的表达显著上调。Munc18b 条件性敲除小鼠中 Muc5ac 的表达没有升高。
结论
肥胖型哮喘中 Muc5ac 的高分泌与 Munc18b 的上调呈正相关。Munc18b 参与诱导 Muc5ac 的高表达。
Zotero 插件