Yu Miao, Guo Danqing, Cao Zhenglin, Xiao Longyi, Wang Gang
a Spine Department , Foshan Hospital of Traditional Chinese Medicine , Foshan, Guangdong , P.R. China.
b Spine Department , The First Affiliated Hospital of Guangzhou University of Chinese Medicine , Guangzhou, Guangdong , P.R. China.
Cancer Invest. 2018 Mar 16;36(3):175-184. doi: 10.1080/07357907.2018.1439055. Epub 2018 Mar 22.
We showed that miR-107 expression was decreased in osteosarcoma (OS) tissues and cell lines. miR-107 mimic significantly decreased OS cell proliferation and inhibited invasion and migration of OS cells. Inhibition of miR-107 expression notably promoted proliferation, invasion and migration of OS cells. In addition, miR-107 mimic inhibited EMT biomarkers and significantly increased apoptosis. miR-107 mimic significantly decreased the protein expression of β-catenin, Cyclin D1, and c-Myc, whereas increased GSK-3β protein expression. miR-107 mimic markedly reduced the luciferase activity of 3'UTR of β-catenin. Overexpression of β-catenin inhibited miR-107 mimic-induced decrease of cell proliferation, invasion and migration ability, and increase of apoptosis.
我们发现骨肉瘤（OS）组织和细胞系中miR-107表达降低。miR-107模拟物显著降低OS细胞增殖，并抑制OS细胞的侵袭和迁移。抑制miR-107表达显著促进OS细胞的增殖、侵袭和迁移。此外，miR-107模拟物抑制上皮-间质转化（EMT）生物标志物并显著增加细胞凋亡。miR-107模拟物显著降低β-连环蛋白、细胞周期蛋白D1和c-Myc的蛋白表达，而增加糖原合成酶激酶-3β（GSK-3β）蛋白表达。miR-107模拟物显著降低β-连环蛋白3'非翻译区（3'UTR）的荧光素酶活性。β-连环蛋白的过表达抑制了miR-107模拟物诱导的细胞增殖、侵袭和迁移能力的降低以及细胞凋亡的增加。
