Wuhan University School of Health Sciences, Wuhan, Hubei Province, 430071, China.
Huangdao District Center for Disease Control and Prevention, Qingdao City, Shandong Province, China.
Ticks Tick Borne Dis. 2018 May;9(4):840-843. doi: 10.1016/j.ttbdis.2018.03.014. Epub 2018 Mar 15.
Anaplasma are tick-borne obligatory intracellular bacteria, which infect humans and other animals. The Anaplasma species carried by ticks in China are not well studied. We collected 3145 questing Haemaphysalis longicornis ticks including 120 larvae, 2460 nymphs and 565 adults from vegetation in Jiaonan County, Shandong Province, China from 2013 to 2015. All ticks were examined for the presence of Anaplasma species by nested PCR amplification of the 16S rRNA gene. For further differentiation of A. capra from A. centrale, gltA and msp2 genes were sequenced for A. capra. Three Anaplasma species were detected in the nymph and/or adult ticks with the minimum infection rate of ticks as follows: 1.55% for A. bovis, 0.10% for A. phagocytophilum, and 0.03% for A. capra. These results indicated that the H. longicornis tick in Jiaonan County carried multiple Anaplasma species, which may be a challenge for public health in the studying area.
恙虫携带的阿纳姆体是蜱传专性细胞内细菌，可感染人类和其他动物。中国蜱携带的阿纳姆体物种尚未得到很好的研究。我们于 2013 年至 2015 年期间，从山东省胶南市植被中采集了 3145 只游离的长角血蜱，包括 120 只幼虫、2460 只若虫和 565 只成虫。通过 16S rRNA 基因嵌套 PCR 扩增，所有蜱都被检查是否存在阿纳姆体物种。为了进一步区分 A. capra 和 A. centrale，对 A. capra 的 gltA 和 msp2 基因进行了测序。在若虫和/或成虫蜱中检测到了三种阿纳姆体物种，其最小感染率如下：1.55%为 A. bovis，0.10%为 A. phagocytophilum，0.03%为 A. capra。这些结果表明，胶南市的长角血蜱携带多种阿纳姆体物种，这可能对研究地区的公共卫生构成挑战。
