Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Talanta. 2018 Jun 1;183:258-267. doi: 10.1016/j.talanta.2018.02.034. Epub 2018 Feb 9.
A highly sensitive electrochemical biosensor based on the synthetized L-Cysteine-Ag(I) coordination polymer (L-Cys-Ag(I) CP), which looks like a protein-mimicking nanowire, was constructed to detect acetylcholinesterase (AChE) activity and screen its inhibitors. This sensing strategy involves the reaction of acetylcholine chloride (ACh) with acetylcholinesterase (AChE) to form choline that is in turn catalytically oxidized by choline oxidase (ChOx) to produce hydrogen peroxide (HO), thus L-Cys-Ag(I) CP possesses the electro-catalytic property to HO reduction. Herein, the protein-mimicking nanowire-based platform was capable of investigating successive of HO effectively by amperometric i-t (current-time) response, and was further applied for the turn-on electrochemical detection of AChE activity. The proposed sensor is highly sensitive (limit of detection is 0.0006 U/L) and is feasible for screening inhibitors of AChE. The model for AChE inhibition was further established and two traditional AChE inhibitors (donepezil and tacrine) were employed to verify the feasibility of the system. The IC0 of donepezil and tacrine were estimated to be 1.4 nM and 3.5 nM, respectively. The developed protocol provides a new and promising platform for probing AChE activity and screening its inhibitors with low cost, high sensitivity and selectivity.
基于合成的 L-半胱氨酸-银（I）配合物（L-Cys-Ag（I）CP）构建了一种高灵敏度的电化学生物传感器，该配合物类似于一种模拟蛋白质的纳米线，用于检测乙酰胆碱酯酶（AChE）活性并筛选其抑制剂。这种传感策略涉及氯化乙酰胆碱（ACh）与乙酰胆碱酯酶（AChE）的反应，形成胆碱，然后胆碱被胆碱氧化酶（ChOx）催化氧化生成过氧化氢（HO），因此 L-Cys-Ag（I）CP 具有对 HO 还原的电催化性能。在此，基于模拟蛋白质的纳米线的平台能够通过安培电流时间（i-t）响应有效地研究 HO 的连续反应，并进一步应用于 AChE 活性的开环电化学检测。该传感器具有高灵敏度（检测限为 0.0006 U/L），可用于筛选 AChE 的抑制剂。进一步建立了 AChE 抑制模型，并采用两种传统的 AChE 抑制剂（多奈哌齐和他克林）验证了该系统的可行性。多奈哌齐和他克林的 IC0 分别估计为 1.4 nM 和 3.5 nM。该开发方案为探测 AChE 活性和筛选其抑制剂提供了一种具有低成本、高灵敏度和选择性的新的有前途的平台。
