Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 48513, Republic of Korea.
Seawell Co., Ltd, Byeoksan e-Centum Classone, 508-1, Centum dong-ro, Haeundae gu, Busan, Republic of Korea.
J Food Drug Anal. 2018 Apr;26(2):545-556. doi: 10.1016/j.jfda.2017.05.006. Epub 2017 Jun 13.
Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) rich 2-monoacylglycerols (2-MAG), omega-3 polyunsaturated free fatty acids (ω-3 PUFFAs) concentrate, and PUFA enriched acylglycerols were prepared from salmon frame bone oil (SFBO) by enzymatic alcoholysis, urea complexation, and enzymatic esterification, respectively. The yields of 2-MAG, ω-3 PUFFAs concentrate, and PUFA enriched acylglycerols were 40.25, 16.52, and 15.65%, respectively. ω-3 PUFFAs concentrate and PUFA enriched acylglycerols showed darker red color than SFBO and 2-MAG due to aggregation of astaxanthin pigment in ω-3 PUFFAs concentrate during urea complexation. The viscosity and specific gravity of SFBO and PUFA enriched acylglycerols showed similar values whereas 2-MAG and ω-3 PUFFAs showed significantly (p < 0.05) lower values. Stability parameters like acid value, peroxide value, free fatty acid value, and p-anisidine value of SFBO and ω-3 PUFAs concentrates were within acceptable limits except extreme high acid value and free fatty acid value of ω-3 PUFFAs concentrate. Thermogravimetric analysis showed similar and higher thermal stability of SFBO and PUFA enriched acylglycerols than 2-MAG and ω-3 PUFFAs concentrate. The ω-3 PUFAs content in 2-MAG, ω-3 PUFFAs concentrate, and PUFA enriched acylglycerols was increased to 20.81, 52.96, and 51.74% respectively from 13.54% in SFBO. ω-3 PUFFAs concentrate and PUFA enriched acylglycerols showed higher DPPH and ABTS radical scavenging activity than SFBO and 2-MAG. The results obtained from this study suggest the production of PUFA enriched acylglycerols rich in ω-3 PUFAs supplements from fish oil for human and pet animals.
从三文鱼下脚料油（SFBO）中通过酶法醇解、尿素包合法和酶法酯化，分别制备出富含 ω-3 多不饱和脂肪酸（ω-3PUFAs）的 2-单酰基甘油（2-MAG）、ω-3 多不饱和脂肪酸浓缩物和富含 PUFA 的甘油酯。2-MAG、ω-3PUFA 浓缩物和富含 PUFA 的甘油酯的产率分别为 40.25%、16.52%和 15.65%。由于 ω-3PUFA 浓缩物在尿素包合过程中虾青素色素聚集，ω-3PUFA 浓缩物和富含 PUFA 的甘油酯的颜色比 SFBO 和 2-MAG 更深。SFBO 和富含 PUFA 的甘油酯的粘度和比重表现出相似的值，而 2-MAG 和 ω-3PUFA 则表现出显著（p<0.05）较低的值。SFBO 和 ω-3PUFA 浓缩物的稳定性参数，如酸值、过氧化值、游离脂肪酸值和对茴香胺值均在可接受范围内，除了 ω-3PUFA 浓缩物的酸值和游离脂肪酸值极高。热重分析表明，SFBO 和富含 PUFA 的甘油酯的热稳定性与 2-MAG 和 ω-3PUFA 浓缩物相似且更高。与 SFBO 中的 13.54%相比，2-MAG、ω-3PUFA 浓缩物和富含 PUFA 的甘油酯中的 ω-3PUFA 含量分别增加到 20.81%、52.96%和 51.74%。ω-3PUFA 浓缩物和富含 PUFA 的甘油酯对 DPPH 和 ABTS 自由基的清除活性均高于 SFBO 和 2-MAG。本研究结果表明，可从鱼油中生产富含 ω-3PUFA 的富含 PUFA 的甘油酯，用于人类和宠物动物的补充剂。
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