Laboratorio de Biología de la Reproducción, Instituto de Fisiología y Biofísica Bernardo Houssay (IFIBIO)- CONICET- Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
Laboratorio de Biología de la Reproducción, Instituto de Fisiología y Biofísica Bernardo Houssay (IFIBIO)- CONICET- Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina; Cátedra de Biología Celular y Molecular, Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Biochem Biophys Res Commun. 2018 May 5;499(2):227-232. doi: 10.1016/j.bbrc.2018.03.133. Epub 2018 Mar 23.
Several aquaporins (AQPs) are expressed in extravillous (EVT) and villous trophoblast cells. Among them, AQP3 is the most abundant AQP expressed in chorionic villi samples from first trimester, followed by AQP1 and AQP9. Although AQP3 expression persists in term placentas, it is significantly decreased in placentas from preeclamptic pregnancies. AQP3 is involved in the migration of different cell types, however its role in human placenta is still unknown. Here, we evaluated the role of AQP3 in the migration of EVT cells during early gestation. Our results showed that Swan 71 cells expressed AQP1, AQP3 and AQP9 but only the blocking of AQP3 by CuSO or the silencing of its expression by siRNA significantly attenuates EVT cell migration. Our work provides evidence that AQP3 is required for EVT cell migration and suggests that an altered expression of placental AQP3 may produce failures in placentation such as in preeclampsia.
几种水通道蛋白 (AQP) 在绒毛外滋养层 (EVT) 和绒毛滋养层细胞中表达。其中，AQP3 在妊娠早期绒毛组织样本中表达最丰富，其次是 AQP1 和 AQP9。尽管 AQP3 在足月胎盘中持续表达，但在子痫前期胎盘中其表达显著降低。AQP3 参与不同细胞类型的迁移，但其在人胎盘中的作用尚不清楚。在这里，我们评估了 AQP3 在妊娠早期 EVT 细胞迁移中的作用。我们的结果表明，Swan 71 细胞表达 AQP1、AQP3 和 AQP9，但只有 AQP3 被 CuSO4 阻断或其表达被 siRNA 沉默才能显著抑制 EVT 细胞迁移。我们的工作提供了证据表明 AQP3 是 EVT 细胞迁移所必需的，并表明胎盘 AQP3 的表达改变可能导致胎盘形成失败，如子痫前期。
