利用近场扫描光学显微镜（NSOM）对蛋白质进行纳米光谱成像。

Nano-spectroscopic imaging of proteins with near-field scanning optical microscopy (NSOM).

机构信息

Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu Province, China.

出版信息

Curr Opin Biotechnol. 2018 Dec;54:106-113. doi: 10.1016/j.copbio.2018.01.022. Epub 2018 Mar 19.

DOI:10.1016/j.copbio.2018.01.022

PMID:29567580

Abstract

Understanding the hierarchical structure of proteins at their fundamental length scales is essential to get insights into their functions and roles in fundamental biological processes. Near-field scanning optical microscopy (NSOM), which overcomes the diffraction limits of conventional optics, provides a powerful analytical tool to image target proteins at nanoscale resolution. Especially, by combining NSOM with infrared (IR) or Raman spectroscopy, near-field nanospectroscopic imaging of a single protein is achieved. In this review, we present the recent technical progress of NSOM setup for nanospectroscopic imaging of proteins, and its application to nanospectroscopic analysis of protein structures is highlighted and critically reviewed. Finally, current challenges and perspectives on application of NSOM in emerging areas of industrial, environmental and medical biotechnology are discussed.

摘要

了解蛋白质在基本长度尺度上的层次结构对于深入了解它们在基本生物过程中的功能和作用至关重要。近场扫描光学显微镜（NSOM）克服了传统光学的衍射限制，为在纳米尺度分辨率下对目标蛋白质进行成像提供了强大的分析工具。特别是，通过将 NSOM 与红外（IR）或拉曼光谱相结合，可以实现单个蛋白质的近场纳米光谱成像。在这篇综述中，我们介绍了 NSOM 用于蛋白质纳米光谱成像的最新技术进展，并重点介绍和批判性地回顾了其在蛋白质结构纳米光谱分析中的应用。最后，讨论了 NSOM 在工业、环境和医疗生物技术等新兴领域的应用所面临的挑战和前景。