Nezammahalleh Hassan, Ghanati Faezeh, Rezaei Shima, Badshah Mohsin Ali, Park Joobee, Abbas Naseem, Ali Ahsan
Faculty of Biological Science, Tarbiat Modares University, Tehran 14115-111, Iran.
Research and Development Department, Hamyarapply Group, Tehran 14115-111, Iran.
Polymers (Basel). 2022 Jul 13;14(14):2853. doi: 10.3390/polym14142853.
Many researchers and scientists have contributed significantly to provide structural and molecular characterizations of biochemical interactions using microscopic techniques in the recent decade, as these biochemical interactions play a crucial role in the production of diverse biomaterials and the organization of biological systems. The properties, activities, and functionalities of the biomaterials and biological systems need to be identified and modified for different purposes in both the material and life sciences. The present study aimed to review the advantages and disadvantages of three main branches of microscopy techniques (optical microscopy, electron microscopy, and scanning probe microscopy) developed for the characterization of these interactions. First, we explain the basic concepts of microscopy and then the breadth of their applicability to different fields of research. This work could be useful for future research works on biochemical self-assembly, biochemical aggregation and localization, biological functionalities, cell viability, live-cell imaging, material stability, and membrane permeability, among others. This understanding is of high importance in rapid, inexpensive, and accurate analysis of biochemical interactions.
近十年来，许多研究人员和科学家利用微观技术在生物化学相互作用的结构和分子表征方面做出了重大贡献，因为这些生物化学相互作用在多种生物材料的生产和生物系统的组织中起着至关重要的作用。在材料科学和生命科学中，出于不同目的，需要对生物材料和生物系统的性质、活性及功能进行识别和修饰。本研究旨在综述为表征这些相互作用而开发的显微镜技术的三个主要分支（光学显微镜、电子显微镜和扫描探针显微镜）的优缺点。首先，我们解释显微镜的基本概念，然后阐述其在不同研究领域的适用范围。这项工作对于未来关于生物化学自组装、生物化学聚集与定位、生物功能、细胞活力、活细胞成像、材料稳定性和膜通透性等方面的研究可能会有所帮助。这种认识对于快速、廉价且准确地分析生物化学相互作用至关重要。
