Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jun 5;198:322-330. doi: 10.1016/j.saa.2018.03.032. Epub 2018 Mar 13.
Simultaneous determination of rosuvastatin calcium and propranolol hydrochloride using the first derivative synchronous spectrofluorimetry was described. This method involves measuring the synchronous fluorescence of both drugs in ethanol using, ∆ λ = 60 nm then the first derivative was recorded and the peak amplitudes were measured at 350 and 374 nm for rosuvastatin calcium and propranolol hydrochloride, respectively. Under the optimum conditions, the linear ranges of rosuvastatin calcium and propranolol hydrochloride were 0.2-2 μg/mL and 0.1-1 μg/mL, respectively. The method was used for quantitative analysis of the drugs in raw materials and pharmaceutical dosage form. The validity of the proposed method was assessed according to an international conference on harmonization (ICH) guidelines.
采用一阶导数同步荧光法同时测定了瑞舒伐他汀钙和盐酸普萘洛尔的含量。该方法采用乙醇作为溶剂，测量两种药物在 ∆ λ = 60nm 处的同步荧光，然后记录一阶导数，分别在 350nm 和 374nm 处测量瑞舒伐他汀钙和盐酸普萘洛尔的峰振幅。在最佳条件下，瑞舒伐他汀钙和盐酸普萘洛尔的线性范围分别为 0.2-2μg/mL 和 0.1-1μg/mL。该方法用于原料药和药物制剂中两种药物的定量分析。所提出的方法的有效性根据国际协调会议（ICH）指南进行了评估。
