Agarwal Preeti, Yadav Sunita, Kumar Archana, Goel Madhu M
Department of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, India.
Department of Pediatrics, King George's Medical University, Lucknow, Uttar Pradesh, India.
J Cytol. 2016 Oct-Dec;33(4):199-204. doi: 10.4103/0970-9371.190454.
Conventional cytology (CC) is a well-established and effective methodology for the evaluation of pediatric tumors. Liquid-based cytology (LBC) is a better technique of smear preparation and is at present the standard protocol in cervical cytology samples.
In the present study, we analyzed both smear preparatory techniques in fine-needle aspiration (FNA) samples from solid pediatric neoplasms in terms of adequacy and efficacy with objectives to study the changes in background and morphology of tumor cells in LBC smears.
This was a prospective observational study conducted in a tertiary care teaching hospital, which included 52 pediatric patients with clinical diagnosis of malignancy and an assessable lump. Both conventional aspiration cytology and LBC smears were prepared as per standard protocols (SurePath BD™) from FNA samples of cases and examined.
On comparing the diagnostic efficacy of cytology smears prepared by both techniques, LBC alone was diagnostic in 80.8% of the cases and conventional smear (CS) alone was diagnostic in 71.2% of the cases (agreement was 83.7%, = 0.3). Cellular morphology was better preserved in LBC and interpretation was easier. There was a lower inadequacy rate in LBC and none of the samples was inadequate due to poor morphology in LBC smears ( = 0.0003). LBC showed an overall 40% improvement in inadequate cases by CS. LBC has been recommended as a complement to CC in nongynecological samples. Improved morphology and lower inadequacy rate make LBC a finer technique compared to CS in pediatric tumors as well.
传统细胞学检查（CC）是评估儿科肿瘤的一种成熟且有效的方法。液基细胞学检查（LBC）是一种更好的涂片制备技术，目前是宫颈细胞学样本的标准方案。
在本研究中，我们从充分性和有效性方面分析了实体儿科肿瘤细针穿刺（FNA）样本的两种涂片制备技术，目的是研究LBC涂片背景和肿瘤细胞形态的变化。
这是一项在三级护理教学医院进行的前瞻性观察研究，纳入了52例临床诊断为恶性肿瘤且有可评估肿块的儿科患者。根据标准方案（SurePath BD™）从病例的FNA样本中制备传统穿刺细胞学涂片和LBC涂片并进行检查。
比较两种技术制备的细胞学涂片的诊断效果，仅LBC诊断出80.8%的病例，仅传统涂片（CS）诊断出71.2%的病例（一致性为83.7%，P = 0.3）。LBC中细胞形态保存更好，解读更容易。LBC的不充分率较低，且没有样本因LBC涂片形态不佳而不充分（P = 0.0003）。LBC显示CS不充分病例总体改善了40%。在非妇科样本中，LBC被推荐作为CC的补充。与CS相比，LBC形态改善且不充分率更低，在儿科肿瘤中也是一种更优良的技术。
