多糖介导的由血清素和其他5-羟基吲哚合成黑色素。
Polysaccharide-mediated synthesis of melanins from serotonin and other 5-hydroxy indoles.
作者信息
Vercruysse Koen, Clark Astiney, Alatas Noor, Brooks Dylan, Hamza Nafisa, Whalen Margaret
机构信息
Department of Chemistry, Tennessee State University, Nashville, TN 37209, USA.
出版信息
Future Sci OA. 2018 Jan 19;4(3):FSO280. doi: 10.4155/fsoa-2017-0118. eCollection 2018 Mar.
AIM
As a continuation of our research on the melanin formation from catecholamines, we studied the polysaccharide-mediated oxidation of serotonin and other 5-hydroxy indoles into melanin-like materials. As for the catecholamines, we observed that many polysaccharides promote the oxidation of such compounds, particularly in the presence of Cu.
METHODOLOGY
The reactions were monitored using reverse phase-HPLC and size-exclusion chromatography techniques. Melanin-like materials were purified through dialysis and characterized using UV-Vis and Fourier transform IR spectroscopic techniques.
RESULTS
One such material, synthesized from chondroitin sulfate type A and serotonin in the presence of Cu was found to affect the release of IL-1β and IL-6 cytokines from immune cells.
目的
作为我们关于儿茶酚胺黑色素形成研究的延续，我们研究了多糖介导的血清素和其他5-羟基吲哚氧化成类黑色素物质的过程。至于儿茶酚胺，我们观察到许多多糖会促进此类化合物的氧化，特别是在有铜存在的情况下。
方法
使用反相高效液相色谱和尺寸排阻色谱技术监测反应。类黑色素物质通过透析纯化，并使用紫外可见光谱和傅里叶变换红外光谱技术进行表征。
结果
发现一种由A型硫酸软骨素和血清素在铜存在下合成的此类物质会影响免疫细胞中白细胞介素-1β和白细胞介素-6细胞因子的释放。
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