Janssens Yorick, Verbeke Frederick, Debunne Nathan, Wynendaele Evelien, Peremans Kathelijne, De Spiegeleer Bart
Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent, Belgium.
Department of Veterinary Medical Imaging and Small Animal Orthopaedics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
J Pharm Anal. 2018 Feb;8(1):69-74. doi: 10.1016/j.jpha.2017.09.001. Epub 2017 Sep 14.
Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ionization (ESI) ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current (TIC) spectra. Non-iodinated peptides and mono- and di-iodinated peptides (NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low (2%) to high (57%).
使用不同的碘化技术对五种不同的群体感应肽（QSP）进行碘化。这些碘化肽使用C反相高效液相色谱系统进行分析，以含有0.1%（m/v）甲酸的水和乙腈线性梯度作为流动相。采用电喷雾电离（ESI）离子阱质谱法鉴定修饰后的肽，同时使用总离子流（TIC）光谱进行半定量。未碘化肽、单碘化肽和双碘化肽（分别为NIP、MIP和DIP）得到了很好的分离，并按此顺序洗脱。根据所使用的碘化方法，碘化产率从低（2%）到高（57%）不等。
