硫酸乙酰肝素和硫酸软骨素合成的抑制与硒酸盐对半翅目昆虫红带锥蝽蜕皮的抑制之间的关系。

A relationship between the inhibition of heparan sulfate and chondroitin sulfate synthesis and the inhibition of molting by selenate in the hemipteran Rhodnius prolixus.

作者信息

Dietrich C P, Nader H B, Toma L, de Azambuja P, Garcia E S

出版信息

Biochem Biophys Res Commun. 1987 Jul 31;146(2):652-8. doi: 10.1016/0006-291x(87)90578-x.

Abstract

The insect Rhodnius prolixus synthesizes heparan sulfate and chondroitin sulfate after a blood meal containing [35S]-inorganic sulfate. A 40 to 80% inhibition of heparan sulfate synthesis was obtained when the meal was supplemented with 10(-5) and 10(-4) M sodium selenate respectively. Likewise an inhibition of the molting in the order of 30 to 60% was observed when the insects were fed with blood containing 10(-5) and 10(-4) M selenate respectively. The insects after a subsequent meal without selenate molted normally. Except for the inhibition of the ecdysis no gross physiological or morphological changes could be observed in the insects. Based on these and other findings the possible role of sulfated glycosaminoglycans in the control of cell growth is discussed.

摘要

昆虫红带锥蝽在摄取含有[35S] - 无机硫酸盐的血餐后会合成硫酸乙酰肝素和硫酸软骨素。当血餐分别添加10^(-5)和10^(-4) M的硒酸钠时，硫酸乙酰肝素的合成受到40%至80%的抑制。同样，当昆虫分别喂食含有10^(-5)和10^(-4) M硒酸盐的血液时，观察到蜕皮抑制率约为30%至60%。在随后摄取不含硒酸盐的血餐后，昆虫正常蜕皮。除了蜕皮受到抑制外，未观察到昆虫有明显的生理或形态变化。基于这些及其他发现，讨论了硫酸化糖胺聚糖在细胞生长控制中的可能作用。