Chihara E, Tsukada-Fujioka T, Tsukahara I
Graefes Arch Clin Exp Ophthalmol. 1984;222(1):45-50. doi: 10.1007/BF02133778.
Cultured chick embryonal retinal pigment epithelial (RPE) cells synthesized two main glycosaminoglycans (GAGs), chondroitin 4-sulfate, and heparan sulfate. Chondroitin 4-sulfate was liberated mainly in the medium (apical side) and heparan sulfate was incorporated into the cell and/or basement membrane. Glycosaminoglycans liberated into the medium were composed of chondroitin 4-sulfate (44%), keratan sulfate (22%), heparan sulfate (18%) and hyaluronate (12%). On the other hand, the GAGs in the cell and/or basement membrane were composed of heparan sulfate (52%), hyaluronate (19%), and chondroitin 4-sulfate (13%).
培养的鸡胚视网膜色素上皮（RPE）细胞合成了两种主要的糖胺聚糖（GAGs），即硫酸软骨素4和硫酸乙酰肝素。硫酸软骨素4主要释放到培养基（顶端侧）中，而硫酸乙酰肝素则整合到细胞和/或基底膜中。释放到培养基中的糖胺聚糖由硫酸软骨素4（44%）、硫酸角质素（22%）、硫酸乙酰肝素（18%）和透明质酸（12%）组成。另一方面，细胞和/或基底膜中的GAGs由硫酸乙酰肝素（52%）、透明质酸（19%）和硫酸软骨素4（13%）组成。
