Ninomiya Y, Hata R, Nigai Y
Biochim Biophys Acta. 1981 Jul;675(2):248-55. doi: 10.1016/0304-4165(81)90234-8.
Rat liver parenchymal cells were evaluated after 2 days of primary culture for their ability to synthesize and accumulate heparan sulfate as the major component and low-sulfated chondroitin sulfate, dermatan sulfate, chondroitin sulfate and hyaluronic acid as the minor ones. The newly synthesized glycosaminoglycans secreted into the medium were different from those remaining with and/or the cell layer. Low-sulfated chondroitin 4-sulfate, a major glycosaminoglycan in blood, was synthesized in the order of 320 microgram/liver per day, more than 90% of which was secreted into the medium with 16 h and 40% of the glycan secreted was degraded during that time. On the other hand, heparan sulfate, the major glycosaminoglycan synthesized by the parenchymal cells, was mainly distributed in the cell layer. After 8 days of culture, the synthesis of glycosaminoglycans by the cells increased markedly, especially dermatan sulfate, chondroitin sulfate, chondroitin sulfate and hyaluronic acid.
原代培养2天后，对大鼠肝实质细胞合成和积累硫酸乙酰肝素（作为主要成分）以及低硫酸化硫酸软骨素、硫酸皮肤素、硫酸软骨素和透明质酸（作为次要成分）的能力进行了评估。分泌到培养基中的新合成糖胺聚糖与留在细胞层和/或与细胞层结合的糖胺聚糖不同。低硫酸化硫酸软骨素4-硫酸酯是血液中的一种主要糖胺聚糖，其合成量约为每天320微克/肝脏，其中90%以上在16小时内分泌到培养基中，且在此期间分泌的聚糖中有40%被降解。另一方面，硫酸乙酰肝素是实质细胞合成的主要糖胺聚糖，主要分布在细胞层中。培养8天后，细胞合成糖胺聚糖的能力显著增加，尤其是硫酸皮肤素、硫酸软骨素、硫酸软骨素和透明质酸。
