Wang Jiabin, Ding Changying, Xiao Jianhua, Jia Wenchao, Chen Yongxuan, Lin Xucong, Xie Zenghong
Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, P. R. China.
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, P. R. China.
Electrophoresis. 2018 Jun;39(12):1504-1511. doi: 10.1002/elps.201800003. Epub 2018 Apr 17.
Via the facile ring-opening reaction of epoxy groups with quinine, a novel polymer monolith with quaternary ammonium for reversed-phase/strong anion-exchange mixed-mode has been fabricated for pressurized capillary electrochromatography (pCEC). Optimization on the preparation of quinine-modified monoliths has been investigated, and characteristics including morphology, permeability, mechanical stability, reproducibility, and column performance have been also studied. Active quaternary ammonium groups were conveniently produced to generate cationic action sites and stable anodic electroosmotic flow. Multiple interactions including reversed-phase, strong anion-exchange, electrostatic repulsion and π-π stacking interactions were obtained. Satisfactory separation capability of various analytes such as alkylbenzenes, polycyclic aromatic hydrocarbons, benzoic acid and its homologs, and β -receptor excitants has been achieved. Applied to the real sample, the good resolution of three alkaloids in Corydalis yanhusuo were achieved by pCEC with the quinine-modified monolith. The results light a potential access to facilely fabricating quaternary ammonium-functionalized polymer monolith with multiple interactions for efficient electrochromatography profiling of various compounds.
通过环氧基团与奎宁的简便开环反应，制备了一种用于加压毛细管电色谱（pCEC）的具有季铵盐的新型反相/强阴离子交换混合模式聚合物整体柱。研究了奎宁改性整体柱制备的优化方法，并对其形态、渗透性、机械稳定性、重现性和柱性能等特性进行了研究。方便地产生了活性季铵基团，以产生阳离子作用位点和稳定的阳极电渗流。获得了包括反相、强阴离子交换、静电排斥和π-π堆积相互作用在内的多种相互作用。实现了对烷基苯、多环芳烃、苯甲酸及其同系物和β-受体兴奋剂等各种分析物的满意分离能力。将其应用于实际样品，采用奎宁改性整体柱的pCEC实现了延胡索中三种生物碱的良好分离。这些结果为简便制备具有多种相互作用的季铵官能化聚合物整体柱以实现各种化合物的高效电色谱分析提供了潜在途径。
