Department of Integrative Marine Ecology , Stazione Zoologica Anton Dohrn , Naples 80121 , Italy.
ACS Appl Mater Interfaces. 2018 Apr 18;10(15):12406-12416. doi: 10.1021/acsami.7b19285. Epub 2018 Apr 4.
In this work, we propose the use of complex, bioderived nanostructures as efficient surface-enhanced Raman scattering (SERS) substrates for chemical analysis of cellular membranes. These structures were directly obtained from a suitable gold metalization of the Pseudonitzchia multistriata diatom silica shell (the so called frustule), whose grating-like geometry provides large light coupling with external radiation, whereas its extruded, subwavelength lateral edge provides an excellent interaction with cells without steric hindrance. We carried out numerical simulations and experimental characterizations of the supported plasmonic resonances and optical near-field amplification. We thoroughly evaluated the SERS substrate enhancement factor as a function of the metalization parameters and finally applied the nanostrucures for discriminating cell membrane Raman signals. In particular, we considered two cases where the membrane composition plays a fundamental role in the assessment of several pathologies, that is, red blood cells and B-leukemia REH cells.
在这项工作中，我们提出使用复杂的生物衍生纳米结构作为有效的表面增强拉曼散射（SERS）基底，用于细胞膜的化学分析。这些结构是直接从合适的 Pseudonitzchia multistriata 硅藻二氧化硅壳（所谓的壳）的金金属化获得的，其光栅状几何形状提供了与外部辐射的大光耦合，而其挤出的亚波长横向边缘提供了与细胞的出色相互作用而不会产生空间障碍。我们进行了支持等离子体共振和光近场放大的数值模拟和实验表征。我们彻底评估了 SERS 基底增强因子作为金属化参数的函数，并最终将纳米结构应用于区分细胞膜拉曼信号。特别是，我们考虑了两种情况，其中膜组成在评估几种病理中起着重要作用，即红细胞和 B 白血病 REH 细胞。
