Servely J L, Geuens G M, Martel P, Houdebine L M, de Brabander M
Biol Cell. 1987;59(2):121-7. doi: 10.1111/j.1768-322x.1987.tb00522.x.
Colchicine and related drugs are known to inhibit milk secretion. They are also able to prevent stimulation of casein and DNA synthesis by prolactin in the mammary gland. The present report reports data obtained with tubulozole, a new antimitotic drug. Tubulozole C added to culture medium of isolated rabbit epithelial mammary cells strongly inhibited their multiplication. Simultaneously, at a concentration of 1 microM, it prevented almost completely the induction of beta-casein mRNA. Induced cells were rapidly deinduced by addition of the drug to the medium. A similar inhibition was observed when the induction was obtained with prolactin alone or with its two stimulators insulin and glucocorticoids. Tubulozole T, an isomer of tubulozole C which is known to be ineffective in disrupting microtubules, did not alter prolactin actions. These data and those obtained with other tubulin-binding drugs strongly suggest that the integrity of microtubules is required for prolactin to deliver its message to the mammary cell.
已知秋水仙碱及相关药物会抑制乳汁分泌。它们还能够阻止催乳素对乳腺中酪蛋白和DNA合成的刺激作用。本报告报道了使用一种新型抗有丝分裂药物tubulozole获得的数据。将tubulozole C添加到分离的兔乳腺上皮细胞培养基中，会强烈抑制其增殖。同时，在浓度为1微摩尔时，它几乎完全阻止了β-酪蛋白mRNA的诱导。向培养基中添加该药物后，诱导细胞迅速去诱导。当单独使用催乳素或其两种刺激物胰岛素和糖皮质激素进行诱导时，也观察到了类似的抑制作用。tubulozole T是tubulozole C的异构体，已知其在破坏微管方面无效，它不会改变催乳素的作用。这些数据以及用其他微管蛋白结合药物获得的数据有力地表明，微管的完整性是催乳素向乳腺细胞传递信息所必需的。
