Cantin M, Ding J, Thibault G, Gutkowska J, Salmi L, Garcia R, Genest J
Mol Cell Endocrinol. 1987 Jul;52(1-2):105-13. doi: 10.1016/0303-7207(87)90103-1.
A comparative study has been made of the amount and form of immunoreactive atrial natriuretic factor (ANF) present in atria versus ventricles in situ and in cultures of atrial and ventricular cardiocytes in rats of various ages: foetus (-2 days of age), newborn (3 days of age), 10 days, 35 days and 70 days old. It was first established that ANF is present in the circulation at all ages investigated, the highest levels being found in the foetus. The atrial content of ANF increased gradually with time and was always in the microgram range. In ventricles, where it was in the nanogram range, total ANF increased with age. More ANF was found in the right than in the left atrium at all ages except in the foetus where the reverse was true. In ventricles, ANF was distributed equally between right ventricle, left ventricle and septum in the foetus, the newborn and in the adult. At 10 days and 35 days, ANF was unequally distributed: (right ventricle greater than septum greater than left ventricle). In cultured atrial cardiocytes, ANF was more abundant than in cultured ventricular cardiocytes at all ages and the amount decreased with the age of the donor animals. Atrial cardiocytes secreted more ANF than did ventricular cardiocytes at all ages investigated, the amount secreted generally decreasing with the age of the donor animals. The secretory activity of atrial cardiocytes per hour was also higher than that of their ventricular counterparts; over this time period, both types of cells secreted more ANF in the presence of serum.(ABSTRACT TRUNCATED AT 250 WORDS)
对不同年龄大鼠（胎儿期 -2 日龄、新生鼠 3 日龄、10 日龄、35 日龄和 70 日龄）的心房和心室原位以及心房和心室心肌细胞培养物中免疫反应性心房利钠因子（ANF）的含量和形式进行了比较研究。首先确定，在所研究的所有年龄段的循环系统中均存在 ANF，胎儿期的水平最高。心房中 ANF 的含量随时间逐渐增加，且始终处于微克范围。心室中的 ANF 处于纳克范围，其总量随年龄增加。除胎儿期情况相反外，所有年龄段右心房中的 ANF 均多于左心房。在胎儿期、新生期和成年期，心室中的 ANF 在右心室、左心室和室间隔中分布均匀。在 10 日龄和 35 日龄时，ANF 分布不均：（右心室＞室间隔＞左心室）。在培养的心房心肌细胞中，所有年龄段的 ANF 均比培养的心室心肌细胞丰富，且其含量随供体动物年龄的增加而减少。在所有研究的年龄段，心房心肌细胞分泌的 ANF 均多于心室心肌细胞，分泌量通常随供体动物年龄的增加而减少。心房心肌细胞每小时的分泌活性也高于心室心肌细胞；在此时间段内，两种类型的细胞在有血清存在的情况下分泌更多的 ANF。（摘要截于 250 字）
