Bloch K D, Seidman J G, Naftilan J D, Fallon J T, Seidman C E
Cell. 1986 Dec 5;47(5):695-702. doi: 10.1016/0092-8674(86)90512-x.
The cellular mechanisms regulating secretion of the peptide hormone atrial natriuretic factor (ANF) differ in neonatal atrial and ventricular cardiocytes. We demonstrate that although both cell types synthesize and secrete ANF, only atrial cells store peptide in abundant secretory granules. Neonatal ventricular cells secrete ANF rapidly after synthesis and lack secretory granules. We propose that ventricular ANF is released by a constitutive secretory pathway whereas atrial ANF is stored and released by a regulated pathway. Furthermore, ventricular ANF mRNA and hormone concentrations decrease during the first week of life. Developmental variation in the use of ANF secretory pathways may reflect changing requirements for maintenance of intravascular volume and pressure. Tissue-specific modulation of hormone secretory pathways appears to be a novel response to developmentally induced changes in the requirements for a peptide hormone.
调节肽类激素心钠素（ANF）分泌的细胞机制在新生心房和心室心肌细胞中有所不同。我们证明，尽管这两种细胞类型都能合成和分泌ANF，但只有心房细胞将肽大量储存在分泌颗粒中。新生心室细胞在合成后迅速分泌ANF，且缺乏分泌颗粒。我们提出，心室ANF通过组成型分泌途径释放，而心房ANF则通过调节型途径储存和释放。此外，心室ANF mRNA和激素浓度在出生后的第一周内会下降。ANF分泌途径使用的发育变化可能反映了维持血管内容量和压力的需求变化。激素分泌途径的组织特异性调节似乎是对肽类激素需求的发育诱导变化的一种新反应。
