Kizheva Yoana, Urshev Zoltan, Rasheva Iliana, Vancheva Taca, Hristova Petya, Bogatzevska Nevena, Moncheva Penka
Department of General and Industrial Microbiology, Faculty of Biology, Sofia University "St. Kliment Ohridski", 8 Dragan Tsankov Str., 1164 Sofia, Bulgaria.
LB Bulgaricum PLC, R&D Center, 12a Malashevska Str., Sofia, Bulgaria.
Z Naturforsch C J Biosci. 2018 Jul 26;73(7-8):257-264. doi: 10.1515/znc-2016-0205.
Pulsed-field gel electrophoresis (PFGE) is a highly discriminative molecular typing method that is used for epidemiological studies and investigation of outbreaks caused by different pathogens, including phytopathogenic Xanthomonas species. Bacterial spot (BS) is the most common and one of the most destructive diseases of tomato and pepper plants in Bulgaria. Several Xanthomonas species are known to cause BS, but the global distribution and genetic diversity of these species are not well understood. A collection of 100 BS-causing strains, isolated during the period of 1985-2012 from different tomato cultivars and weeds associated with tomato production areas from 11 geographic regions in Bulgaria, were screened for genetic diversity by genomic DNA restriction with rare-cutting endonucleases (XbaI and SpeI) subsequently resolved by PFGE. Two haplotypes for Xanthomonas vesicatoria and one haplotype for Xanthomonas gardneri strains were found.
脉冲场凝胶电泳（PFGE）是一种具有高度鉴别力的分子分型方法，用于流行病学研究以及调查由不同病原体引起的疫情爆发，包括植物致病黄单胞菌属物种。细菌性斑点病（BS）是保加利亚番茄和辣椒植株最常见且最具破坏性的病害之一。已知有几种黄单胞菌属物种会引发细菌性斑点病，但这些物种的全球分布和遗传多样性尚未得到充分了解。对1985年至2012年期间从保加利亚11个地理区域与番茄生产区相关的不同番茄品种和杂草中分离出的100株引发细菌性斑点病的菌株进行了收集，通过用稀有切割内切酶（XbaI和SpeI）对基因组DNA进行限制，随后通过PFGE进行分析，以筛选其遗传多样性。发现了水泡性黄单胞菌的两种单倍型和加德纳氏黄单胞菌菌株的一种单倍型。
