Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, 510006, PR China.
Department of Food Science and Engineering, Institute of Food Safety and Nutrition, Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection, Jinan University, Guangzhou, 510632, PR China.
Food Chem Toxicol. 2018 Sep;119:268-274. doi: 10.1016/j.fct.2018.03.027. Epub 2018 Mar 21.
Acrylamide (AA) occurs in many cooked carbohydrate-rich foods and has caused widespread concern as a possible carcinogen. Glycidamide (GA) is the ultimate genotoxic metabolite of AA. The present study was to investigate the protective effect of Cyanidin-3-O-glucoside (C3G) against AA- and GA-induced reproductive toxicity in R2C Leydig cells. The results demonstrated that C3G inhibited AA- and GA-induced cytotoxicity and mitochondria-mediated cell apoptosis, the effective doses of C3G were ranging from 10 to 50 μM. Besides, AA (1.925 mM) and GA (0.872 mM) exposure increased ROS level and decreased mitochondrial membrane potential, which led to a decrease in progesterone production, while C3G ranging from 10 to 50 μM reduced ROS immediately, and increased progesterone production after 24 h treatment. Furthermore, C3G up-regulated expression of Bcl-2 protein and down-regulated pro-apoptotic protein Bax and cleaved Caspase-3 after 24 h treatment in 1.925 mM AA- and 0.872 mM GA-treated R2C cells. Moreover, C3G intervention increased the protein expression of steroidogenic acute regulatory (StAR). It was concluded that C3G is effective in reducing AA- and GA-induced reproductive toxicity via inhibition of ROS generation, mitochondrial membrane depolarization and apoptosis, as well as activating steroidogenic enzymes.
丙烯酰胺（AA）存在于许多烹饪的富含碳水化合物的食物中，作为一种可能的致癌物质引起了广泛关注。丙烯酰胺的最终遗传毒性代谢物是缩水甘油酰胺（GA）。本研究旨在探讨矢车菊素-3-O-葡萄糖苷（C3G）对 R2C 莱迪希细胞中 AA 和 GA 诱导的生殖毒性的保护作用。结果表明，C3G 抑制 AA 和 GA 诱导的细胞毒性和线粒体介导的细胞凋亡，C3G 的有效剂量范围为 10 至 50 μM。此外，AA（1.925 mM）和 GA（0.872 mM）暴露增加了 ROS 水平并降低了线粒体膜电位，导致孕激素产生减少，而 10 至 50 μM 的 C3G 立即降低 ROS，并在 24 小时处理后增加孕激素产生。此外，C3G 在 1.925 mM AA 和 0.872 mM GA 处理的 R2C 细胞中，24 小时后上调 Bcl-2 蛋白的表达，并下调促凋亡蛋白 Bax 和切割的 Caspase-3。此外，C3G 干预增加了类固醇生成急性调节蛋白（StAR）的蛋白表达。总之，C3G 通过抑制 ROS 生成、线粒体膜去极化和细胞凋亡，以及激活甾体生成酶，有效降低 AA 和 GA 诱导的生殖毒性。
