PolymerEngineering Laboratory, Indian Institute of Technology, Banaras Hindu University, Varanasi, 221005, India.
Tissue Engineering and Biomicrofluidics Laboratory, School of Biomedical Engineering, Indian Institute of Technology, Banaras Hindu University, Varanasi, 221005, India.
Colloids Surf B Biointerfaces. 2018 Jun 1;166:170-178. doi: 10.1016/j.colsurfb.2018.03.014. Epub 2018 Mar 14.
Surface and mechanical properties of the biomaterials are determinants of cellular responses. In our previous study, star-shaped poly(d,l-Lactide)-b-gelatin (ss-pLG) was reported for possessing improved cellular adhesion and proliferation. Here, we extended our investigation to establish the cellular compatibility of gelatin-grafted PDLLA with respect to mechanical properties of biological tissues. In this view, linear PDLLA-b-gelatin (l-pLG) was synthesized and tissue-level compatibility of 1-pLG and ss-pLG against fibroblasts (L929), myoblasts (C2C12) and preosteoblasts (MG-63) was examined. The cell proliferation of C2C12 was significantly higher within l-pLG scaffolds, whereas L929 showed intensified growth within ss-pLG scaffolds. The difference in cell proliferation may be attributed to the varying mechanical properties of scaffolds; where the stiffness of l-pLG scaffolds was notably higher than ss-pLG scaffolds, most likely due to the variable levels of gelatin grafting on the backbone of PDLLA. Therefore, gelatin grafting can be used to modulate mechanical property of the scaffolds and this study reveals the significance of the matrix stiffness to produce the successful 3D scaffolds for tissue engineering applications.
生物材料的表面和机械性能是细胞反应的决定因素。在我们之前的研究中，星形聚（D，L-丙交酯）-b-明胶（ss-pLG）被报道具有改善的细胞黏附和增殖。在这里，我们扩展了研究范围，以确定明胶接枝 PDLLA 相对于组织机械性能的细胞相容性。在这种情况下，合成了线性 PDLLA-b-明胶（l-pLG），并对成纤维细胞（L929）、成肌细胞（C2C12）和前成骨细胞（MG-63）的 1-pLG 和 ss-pLG 的组织相容性进行了研究。C2C12 的细胞增殖在 l-pLG 支架内显著升高，而 L929 在 ss-pLG 支架内表现出增强的生长。细胞增殖的差异可能归因于支架机械性能的变化；l-pLG 支架的硬度明显高于 ss-pLG 支架，这可能是由于 PDLLA 主链上接枝的明胶水平不同。因此，明胶接枝可用于调节支架的机械性能，本研究揭示了基质硬度对成功制备用于组织工程应用的 3D 支架的重要性。
